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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1264 | 5' | -54.1 | NC_001317.1 | + | 639 | 0.7 | 0.3569 |
|
Target: 5'- ---uGCggCGGaaCAGCGUUGCGGAUUCa -3' miRNA: 3'- caguCGaaGCC--GUCGCAGUGCCUAAG- -5' |
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| 1264 | 5' | -54.1 | NC_001317.1 | + | 1772 | 0.7 | 0.3569 |
|
Target: 5'- uGUCAGCgcaUCGaGCAGCGccUCACGGc--- -3' miRNA: 3'- -CAGUCGa--AGC-CGUCGC--AGUGCCuaag -5' |
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| 1264 | 5' | -54.1 | NC_001317.1 | + | 3625 | 0.66 | 0.588483 |
|
Target: 5'- -gCAGCauUUCGGCAaucuGCGgCACGGAa-- -3' miRNA: 3'- caGUCG--AAGCCGU----CGCaGUGCCUaag -5' |
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| 1264 | 5' | -54.1 | NC_001317.1 | + | 8463 | 0.71 | 0.313829 |
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Target: 5'- --gAGCUguUCGcGCAGUGUCaccugcGCGGAUUCa -3' miRNA: 3'- cagUCGA--AGC-CGUCGCAG------UGCCUAAG- -5' |
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| 1264 | 5' | -54.1 | NC_001317.1 | + | 15487 | 1.1 | 0.000448 |
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Target: 5'- gGUCAGCUUCGGCAGCGUCACGGAUUCa -3' miRNA: 3'- -CAGUCGAAGCCGUCGCAGUGCCUAAG- -5' |
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| 1264 | 5' | -54.1 | NC_001317.1 | + | 19759 | 0.69 | 0.380882 |
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Target: 5'- --aAGCgcaauaaacugaCGGcCAGUGUCGCGGAUUCa -3' miRNA: 3'- cagUCGaa----------GCC-GUCGCAGUGCCUAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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