Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1265 | 3' | -56.7 | NC_001317.1 | + | 23731 | 0.67 | 0.356787 |
Target: 5'- cGAGCGguGCGGUaucugAUGaGCGUCugaccacuGCCCg -3' miRNA: 3'- uUUUGCguCGCCG-----UGC-CGUAGu-------CGGG- -5' |
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1265 | 3' | -56.7 | NC_001317.1 | + | 18753 | 0.69 | 0.290545 |
Target: 5'- gGAggUGCuGCGcCGgGGCAUCAGCUUg -3' miRNA: 3'- -UUuuGCGuCGCcGUgCCGUAGUCGGG- -5' |
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1265 | 3' | -56.7 | NC_001317.1 | + | 17847 | 0.66 | 0.44311 |
Target: 5'- uAAACGCAGCGGUgagcCGGUcaaGUUuGCCg -3' miRNA: 3'- uUUUGCGUCGCCGu---GCCG---UAGuCGGg -5' |
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1265 | 3' | -56.7 | NC_001317.1 | + | 16086 | 0.77 | 0.077782 |
Target: 5'- cGAACGCAgGCacccuGCGCGGCGUCAGUCUg -3' miRNA: 3'- uUUUGCGU-CGc----CGUGCCGUAGUCGGG- -5' |
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1265 | 3' | -56.7 | NC_001317.1 | + | 15655 | 1.07 | 0.000377 |
Target: 5'- cAAACGCAGCGGCACGGCAUCAGCCCc -3' miRNA: 3'- uUUUGCGUCGCCGUGCCGUAGUCGGG- -5' |
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1265 | 3' | -56.7 | NC_001317.1 | + | 15475 | 0.67 | 0.368505 |
Target: 5'- ---uCGCGGCGGCGguaugaaUGGCGcugcagcgauugaccUCGGCCUc -3' miRNA: 3'- uuuuGCGUCGCCGU-------GCCGU---------------AGUCGGG- -5' |
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1265 | 3' | -56.7 | NC_001317.1 | + | 14834 | 0.71 | 0.209821 |
Target: 5'- cAAAACGCugucuaacguuGGCGuGCAgggcguuacCGGCAUCAGCgCCu -3' miRNA: 3'- -UUUUGCG-----------UCGC-CGU---------GCCGUAGUCG-GG- -5' |
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1265 | 3' | -56.7 | NC_001317.1 | + | 14513 | 0.66 | 0.431931 |
Target: 5'- cAAGGCGCugcuaacugccgaAGCGGuCAcCGGCGUUaAGCCg -3' miRNA: 3'- -UUUUGCG-------------UCGCC-GU-GCCGUAG-UCGGg -5' |
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1265 | 3' | -56.7 | NC_001317.1 | + | 9913 | 0.67 | 0.365778 |
Target: 5'- cGAACGac-CGGCGCGGgugGUCAGCCa -3' miRNA: 3'- uUUUGCgucGCCGUGCCg--UAGUCGGg -5' |
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1265 | 3' | -56.7 | NC_001317.1 | + | 9491 | 0.67 | 0.384222 |
Target: 5'- cAAggUGCAGCgcauGGCGCGgguGCAUCAGUa- -3' miRNA: 3'- -UUuuGCGUCG----CCGUGC---CGUAGUCGgg -5' |
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1265 | 3' | -56.7 | NC_001317.1 | + | 8940 | 0.68 | 0.306155 |
Target: 5'- ---cCGguGCaGGCAUGGCugcgugaacAUCAGCCg -3' miRNA: 3'- uuuuGCguCG-CCGUGCCG---------UAGUCGGg -5' |
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1265 | 3' | -56.7 | NC_001317.1 | + | 8823 | 0.73 | 0.13674 |
Target: 5'- ----aGCuGCGGCACGGCGuuaaUCAGCgCu -3' miRNA: 3'- uuuugCGuCGCCGUGCCGU----AGUCGgG- -5' |
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1265 | 3' | -56.7 | NC_001317.1 | + | 8736 | 0.67 | 0.347953 |
Target: 5'- ---cUGCAGCGcGCA-GGCAgUCAGCgCCc -3' miRNA: 3'- uuuuGCGUCGC-CGUgCCGU-AGUCG-GG- -5' |
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1265 | 3' | -56.7 | NC_001317.1 | + | 7021 | 0.69 | 0.261212 |
Target: 5'- uAAACcCGGCGGCAgucUGGCG-CAGCCg -3' miRNA: 3'- uUUUGcGUCGCCGU---GCCGUaGUCGGg -5' |
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1265 | 3' | -56.7 | NC_001317.1 | + | 6430 | 0.68 | 0.314197 |
Target: 5'- uGAAUGCAGaGGCGCuGGCGcaguuUCAGCgCg -3' miRNA: 3'- uUUUGCGUCgCCGUG-CCGU-----AGUCGgG- -5' |
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1265 | 3' | -56.7 | NC_001317.1 | + | 6001 | 0.67 | 0.351468 |
Target: 5'- -uGGCGCAGCGgggcuuguucccgcuGCGCGGCuugagaGGCCg -3' miRNA: 3'- uuUUGCGUCGC---------------CGUGCCGuag---UCGGg -5' |
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1265 | 3' | -56.7 | NC_001317.1 | + | 1732 | 0.66 | 0.422909 |
Target: 5'- -uAACcuuuCGGCGcGCGuCGGCGUCAGUCUu -3' miRNA: 3'- uuUUGc---GUCGC-CGU-GCCGUAGUCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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