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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 1268 | 3' | -59.3 | NC_001317.1 | + | 17774 | 0.66 | 0.334244 |
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Target: 5'- aAGCCGCCUCaa--GCGGCAAacuugacCGGCu -3' miRNA: 3'- aUCGGCGGGGgugaCGCUGUUa------GCCG- -5' |
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| 1268 | 3' | -59.3 | NC_001317.1 | + | 16981 | 0.66 | 0.342622 |
|
Target: 5'- aUGGCCGCagCaggCGCUGCGG-GGUCGGUa -3' miRNA: 3'- -AUCGGCGg-Gg--GUGACGCUgUUAGCCG- -5' |
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| 1268 | 3' | -59.3 | NC_001317.1 | + | 7266 | 0.66 | 0.334244 |
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Target: 5'- gAGCUGCCCCaCAaUGCaGCg--CGGCu -3' miRNA: 3'- aUCGGCGGGG-GUgACGcUGuuaGCCG- -5' |
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| 1268 | 3' | -59.3 | NC_001317.1 | + | 19913 | 0.68 | 0.238998 |
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Target: 5'- -cGUCG-CCCCAUUGCGGucagcCAGUCGGg -3' miRNA: 3'- auCGGCgGGGGUGACGCU-----GUUAGCCg -5' |
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| 1268 | 3' | -59.3 | NC_001317.1 | + | 23205 | 0.69 | 0.197783 |
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Target: 5'- --aCCGCCUaCCGCUG-GACAAcCGGCg -3' miRNA: 3'- aucGGCGGG-GGUGACgCUGUUaGCCG- -5' |
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| 1268 | 3' | -59.3 | NC_001317.1 | + | 20825 | 0.7 | 0.167526 |
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Target: 5'- aUGGCCGCUgaCCAC--CGACAGUCGGg -3' miRNA: 3'- -AUCGGCGGg-GGUGacGCUGUUAGCCg -5' |
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| 1268 | 3' | -59.3 | NC_001317.1 | + | 18262 | 1.09 | 0.000169 |
|
Target: 5'- aUAGCCGCCCCCACUGCGACAAUCGGCc -3' miRNA: 3'- -AUCGGCGGGGGUGACGCUGUUAGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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