Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12683 | 3' | -61.8 | NC_003345.1 | + | 24132 | 0.69 | 0.354131 |
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Target: 5'- uGUUGuccCCGUCGuCCgUGUGGCCCCg -3' miRNA: 3'- cCGACuu-GGCGGCuGGaGCGCCGGGG- -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 51869 | 0.68 | 0.386337 |
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Target: 5'- aGGUUG-ACC-CCGACUUCGgGGCUggaaCCg -3' miRNA: 3'- -CCGACuUGGcGGCUGGAGCgCCGG----GG- -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 42515 | 0.67 | 0.426565 |
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Target: 5'- cGGCUGAAucggucgcgggUCGCCggacugauuuuuguGACCUCGCccGUCCCc -3' miRNA: 3'- -CCGACUU-----------GGCGG--------------CUGGAGCGc-CGGGG- -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 57938 | 0.66 | 0.474774 |
|
Target: 5'- aGGCUacugGAAUCGaCC-ACUUgGCGGCuCCCg -3' miRNA: 3'- -CCGA----CUUGGC-GGcUGGAgCGCCG-GGG- -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 19861 | 0.66 | 0.484175 |
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Target: 5'- aGCaGAuaACCGCCG-CUggCGCGGCUCa -3' miRNA: 3'- cCGaCU--UGGCGGCuGGa-GCGCCGGGg -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 72423 | 0.78 | 0.088683 |
|
Target: 5'- cGCUgGAGCCGCCGGgCggcgGUGGCCCCg -3' miRNA: 3'- cCGA-CUUGGCGGCUgGag--CGCCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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