Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12683 | 3' | -61.8 | NC_003345.1 | + | 46128 | 0.66 | 0.474774 |
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Target: 5'- cGCaUGAcgcACCGCgCGACCUCGUagGaGUCCUg -3' miRNA: 3'- cCG-ACU---UGGCG-GCUGGAGCG--C-CGGGG- -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 19861 | 0.66 | 0.484175 |
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Target: 5'- aGCaGAuaACCGCCG-CUggCGCGGCUCa -3' miRNA: 3'- cCGaCU--UGGCGGCuGGa-GCGCCGGGg -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 32446 | 0.66 | 0.493665 |
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Target: 5'- cGGCcuugggGAACgcuuccccgaCGCCGACCUCgauGCGGUgagccaCCCg -3' miRNA: 3'- -CCGa-----CUUG----------GCGGCUGGAG---CGCCG------GGG- -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 42308 | 0.66 | 0.503239 |
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Target: 5'- uGgUGGACUGaCGGCUUCGCGGagcaaaugaCCCg -3' miRNA: 3'- cCgACUUGGCgGCUGGAGCGCCg--------GGG- -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 14235 | 0.66 | 0.521646 |
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Target: 5'- aGGCaGAACCGCCucuuGACUUCGauGaagugcuggaaaaCCCCg -3' miRNA: 3'- -CCGaCUUGGCGG----CUGGAGCgcC-------------GGGG- -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 63461 | 0.66 | 0.532422 |
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Target: 5'- gGGCUGu-CCucaCCGAaccaCUCGCGGgCCUu -3' miRNA: 3'- -CCGACuuGGc--GGCUg---GAGCGCCgGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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