Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12683 | 3' | -61.8 | NC_003345.1 | + | 73723 | 0.73 | 0.201533 |
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Target: 5'- gGGCgcgGAGCCGCUGACgacuaCUCcuaCGGCCUCa -3' miRNA: 3'- -CCGa--CUUGGCGGCUG-----GAGc--GCCGGGG- -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 45349 | 0.75 | 0.145363 |
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Target: 5'- aGCUGAACCGCCGccuUCUCGCucaccucGCCCUu -3' miRNA: 3'- cCGACUUGGCGGCu--GGAGCGc------CGGGG- -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 25337 | 0.75 | 0.145363 |
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Target: 5'- cGGCUGAacaagACCGCUGGuucucUCUCGCGGCgUCu -3' miRNA: 3'- -CCGACU-----UGGCGGCU-----GGAGCGCCGgGG- -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 25680 | 0.75 | 0.14133 |
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Target: 5'- aGCUGAACUucaacgaGCagguaGACCUCGCGGCUCa -3' miRNA: 3'- cCGACUUGG-------CGg----CUGGAGCGCCGGGg -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 72423 | 0.78 | 0.088683 |
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Target: 5'- cGCUgGAGCCGCCGGgCggcgGUGGCCCCg -3' miRNA: 3'- cCGA-CUUGGCGGCUgGag--CGCCGGGG- -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 75304 | 1.13 | 0.000229 |
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Target: 5'- gGGCUGAACCGCCGACCUCGCGGCCCCg -3' miRNA: 3'- -CCGACUUGGCGGCUGGAGCGCCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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