Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1269 | 3' | -50.4 | NC_001317.1 | + | 15218 | 0.66 | 0.838106 |
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Target: 5'- aCCCUCaagGCCGGGAAACUGuAUAu----- -3' miRNA: 3'- -GGGAG---CGGCUCUUUGAC-UAUucgucc -5' |
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| 1269 | 3' | -50.4 | NC_001317.1 | + | 10042 | 0.67 | 0.788137 |
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Target: 5'- aUC-CGCCGAGGcaGACg---GAGCGGGg -3' miRNA: 3'- gGGaGCGGCUCU--UUGacuaUUCGUCC- -5' |
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| 1269 | 3' | -50.4 | NC_001317.1 | + | 1033 | 0.67 | 0.766762 |
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Target: 5'- cUCUUUGCCagcccgGAGAAGCUGAccGGCGGc -3' miRNA: 3'- -GGGAGCGG------CUCUUUGACUauUCGUCc -5' |
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| 1269 | 3' | -50.4 | NC_001317.1 | + | 9677 | 0.67 | 0.766762 |
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Target: 5'- -aUUUGCCGAGggGCUGAUAAu---- -3' miRNA: 3'- ggGAGCGGCUCuuUGACUAUUcgucc -5' |
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| 1269 | 3' | -50.4 | NC_001317.1 | + | 18335 | 0.68 | 0.75583 |
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Target: 5'- gCCCUCaaucacccCCGAGAAaaaugcGCUGAUGGGCu-- -3' miRNA: 3'- -GGGAGc-------GGCUCUU------UGACUAUUCGucc -5' |
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| 1269 | 3' | -50.4 | NC_001317.1 | + | 1391 | 0.69 | 0.6877 |
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Target: 5'- gCUgUCGCCGGu--AUUGAUGAGCGGu -3' miRNA: 3'- -GGgAGCGGCUcuuUGACUAUUCGUCc -5' |
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| 1269 | 3' | -50.4 | NC_001317.1 | + | 1610 | 0.73 | 0.458823 |
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Target: 5'- uUCCUCGCCGAcaAAGCUGAUAAccGCAc- -3' miRNA: 3'- -GGGAGCGGCUc-UUUGACUAUU--CGUcc -5' |
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| 1269 | 3' | -50.4 | NC_001317.1 | + | 17821 | 0.75 | 0.350762 |
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Target: 5'- gCCUCGgacgguaaaaCCGAGGAACUGGUuaagguuauGCAGGa -3' miRNA: 3'- gGGAGC----------GGCUCUUUGACUAuu-------CGUCC- -5' |
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| 1269 | 3' | -50.4 | NC_001317.1 | + | 19039 | 1.13 | 0.000962 |
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Target: 5'- cCCCUCGCCGAGAAACUGAUAAGCAGGc -3' miRNA: 3'- -GGGAGCGGCUCUUUGACUAUUCGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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