Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12725 | 5' | -51.5 | NC_003356.1 | + | 34230 | 0.66 | 0.852111 |
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Target: 5'- uCAUACCGGCAGAaaCAG-CAGaauuaACCa -3' miRNA: 3'- cGUAUGGUUGUCUgaGUCgGUCg----UGG- -5' |
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| 12725 | 5' | -51.5 | NC_003356.1 | + | 34798 | 0.68 | 0.730331 |
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Target: 5'- aGCGaacGCCAGCGGAagUUCuGCCuGCGCUu -3' miRNA: 3'- -CGUa--UGGUUGUCU--GAGuCGGuCGUGG- -5' |
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| 12725 | 5' | -51.5 | NC_003356.1 | + | 36846 | 0.7 | 0.581394 |
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Target: 5'- ---gACCGggACAGACUC-GCUGGCACUu -3' miRNA: 3'- cguaUGGU--UGUCUGAGuCGGUCGUGG- -5' |
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| 12725 | 5' | -51.5 | NC_003356.1 | + | 38090 | 0.71 | 0.547259 |
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Target: 5'- -gAUACCGACGGugUacuugaCGGUCuGGCACCa -3' miRNA: 3'- cgUAUGGUUGUCugA------GUCGG-UCGUGG- -5' |
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| 12725 | 5' | -51.5 | NC_003356.1 | + | 39340 | 0.7 | 0.597483 |
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Target: 5'- gGCAUAaacauCCAGCAGGCcgucaccauucuguuUCAgGCCGGUAUCg -3' miRNA: 3'- -CGUAU-----GGUUGUCUG---------------AGU-CGGUCGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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