miRNA display CGI


Results 21 - 25 of 25 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12725 5' -51.5 NC_003356.1 + 25609 0.66 0.833839
Target:  5'- gGCGcUGCCuGCGuuGCUCuGgCAGCACCg -3'
miRNA:   3'- -CGU-AUGGuUGUc-UGAGuCgGUCGUGG- -5'
12725 5' -51.5 NC_003356.1 + 9618 0.66 0.833839
Target:  5'- gGCAggggaGCCGAaAGGCUC-GCCgguuuccuugAGCGCCg -3'
miRNA:   3'- -CGUa----UGGUUgUCUGAGuCGG----------UCGUGG- -5'
12725 5' -51.5 NC_003356.1 + 13452 0.66 0.833839
Target:  5'- -aAUG-CAGCAGcCUCAGCC-GCGCa -3'
miRNA:   3'- cgUAUgGUUGUCuGAGUCGGuCGUGg -5'
12725 5' -51.5 NC_003356.1 + 29457 0.66 0.833839
Target:  5'- uGCuguauUugCAGCAGAauuguccauCUCGGCguaaaacaaCGGCACCa -3'
miRNA:   3'- -CGu----AugGUUGUCU---------GAGUCG---------GUCGUGG- -5'
12725 5' -51.5 NC_003356.1 + 34230 0.66 0.852111
Target:  5'- uCAUACCGGCAGAaaCAG-CAGaauuaACCa -3'
miRNA:   3'- cGUAUGGUUGUCUgaGUCgGUCg----UGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.