Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12725 | 5' | -51.5 | NC_003356.1 | + | 24173 | 0.74 | 0.399654 |
Target: 5'- ---aGCCGGgGGAUaaaUCAGCCAGgCACCa -3' miRNA: 3'- cguaUGGUUgUCUG---AGUCGGUC-GUGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 19075 | 0.74 | 0.38069 |
Target: 5'- cGCAguagagGCCAgaGCAcGGC-CAGCaCAGCACCa -3' miRNA: 3'- -CGUa-----UGGU--UGU-CUGaGUCG-GUCGUGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 24319 | 0.74 | 0.380689 |
Target: 5'- cUAUACCGgaaaGCGGAUcagCAGCCAGCggGCCa -3' miRNA: 3'- cGUAUGGU----UGUCUGa--GUCGGUCG--UGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 21473 | 0.75 | 0.353393 |
Target: 5'- uGCAgcACCAgcGCGGACUgAGCCGcCACCa -3' miRNA: 3'- -CGUa-UGGU--UGUCUGAgUCGGUcGUGG- -5' |
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12725 | 5' | -51.5 | NC_003356.1 | + | 24453 | 1.14 | 0.000737 |
Target: 5'- aGCAUACCAACAGACUCAGCCAGCACCc -3' miRNA: 3'- -CGUAUGGUUGUCUGAGUCGGUCGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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