Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12730 | 3' | -53 | NC_003356.1 | + | 32572 | 0.66 | 0.762926 |
Target: 5'- -aUAuGCUGC-CCGUGCCGCgaCGACGg -3' miRNA: 3'- gaAUuUGACGcGGCGCGGUG--GUUGUg -5' |
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12730 | 3' | -53 | NC_003356.1 | + | 1277 | 0.66 | 0.741284 |
Target: 5'- aUUAAACcagaUGCGUaaUGUGCCACCGugGu -3' miRNA: 3'- gAAUUUG----ACGCG--GCGCGGUGGUugUg -5' |
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12730 | 3' | -53 | NC_003356.1 | + | 3000 | 0.66 | 0.741284 |
Target: 5'- -cUGAcCUGCGCCagaaGaUCGCCGACGCa -3' miRNA: 3'- gaAUUuGACGCGGcg--C-GGUGGUUGUG- -5' |
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12730 | 3' | -53 | NC_003356.1 | + | 31434 | 0.66 | 0.734692 |
Target: 5'- -----uCUGCGCCGCcGaugaacgcgggauccCCGCCAGCAg -3' miRNA: 3'- gaauuuGACGCGGCG-C---------------GGUGGUUGUg -5' |
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12730 | 3' | -53 | NC_003356.1 | + | 15992 | 0.66 | 0.719159 |
Target: 5'- ---uGGCUG-GCagGUGCCGCUAACACc -3' miRNA: 3'- gaauUUGACgCGg-CGCGGUGGUUGUG- -5' |
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12730 | 3' | -53 | NC_003356.1 | + | 27017 | 0.67 | 0.673881 |
Target: 5'- -aUGAACUucGCGCCGCuGCggugCGCCuuGCGCa -3' miRNA: 3'- gaAUUUGA--CGCGGCG-CG----GUGGu-UGUG- -5' |
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12730 | 3' | -53 | NC_003356.1 | + | 35288 | 0.67 | 0.650932 |
Target: 5'- -gUGAACUG-GCUGCGCCAUuUGGCAUc -3' miRNA: 3'- gaAUUUGACgCGGCGCGGUG-GUUGUG- -5' |
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12730 | 3' | -53 | NC_003356.1 | + | 33423 | 0.68 | 0.615244 |
Target: 5'- aCUUAAGgUGaCGUuugacaaCGCGCCGCCGggaauGCGCg -3' miRNA: 3'- -GAAUUUgAC-GCG-------GCGCGGUGGU-----UGUG- -5' |
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12730 | 3' | -53 | NC_003356.1 | + | 24252 | 0.68 | 0.598011 |
Target: 5'- --cAGACUGcCGUguacggucgagauauCGUGCCACCAGcCACg -3' miRNA: 3'- gaaUUUGAC-GCG---------------GCGCGGUGGUU-GUG- -5' |
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12730 | 3' | -53 | NC_003356.1 | + | 34598 | 0.75 | 0.246187 |
Target: 5'- --cAGACUGCGCUcaGCagaGCCGCCAACAg -3' miRNA: 3'- gaaUUUGACGCGG--CG---CGGUGGUUGUg -5' |
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12730 | 3' | -53 | NC_003356.1 | + | 40554 | 0.77 | 0.19202 |
Target: 5'- ----uGCUGCGCCuGCGCCACCAu--- -3' miRNA: 3'- gaauuUGACGCGG-CGCGGUGGUugug -5' |
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12730 | 3' | -53 | NC_003356.1 | + | 28724 | 1.11 | 0.000785 |
Target: 5'- cCUUAAACUGCGCCGCGCCACCAACACg -3' miRNA: 3'- -GAAUUUGACGCGGCGCGGUGGUUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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