Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12731 | 5' | -62 | NC_003356.1 | + | 13376 | 0.67 | 0.2718 |
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Target: 5'- -aUGCCCCuggCCUGCGuAUCCGGguuguacacuuCAUCCc -3' miRNA: 3'- gaGCGGGG---GGACGCcUAGGCC-----------GUAGG- -5' |
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| 12731 | 5' | -62 | NC_003356.1 | + | 32256 | 0.68 | 0.25867 |
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Target: 5'- gUUGCCCCCaUUGCGGcaaucucUCCGGaggCCa -3' miRNA: 3'- gAGCGGGGG-GACGCCu------AGGCCguaGG- -5' |
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| 12731 | 5' | -62 | NC_003356.1 | + | 37574 | 0.73 | 0.109798 |
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Target: 5'- -gCGCCCCCCUGUGGAauguaguaauaacguUCCauaacgcgGGCAcgCCa -3' miRNA: 3'- gaGCGGGGGGACGCCU---------------AGG--------CCGUa-GG- -5' |
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| 12731 | 5' | -62 | NC_003356.1 | + | 22329 | 0.74 | 0.094665 |
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Target: 5'- aUCGCUCCCCUGC---UUCGGCGUCa -3' miRNA: 3'- gAGCGGGGGGACGccuAGGCCGUAGg -5' |
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| 12731 | 5' | -62 | NC_003356.1 | + | 31790 | 0.8 | 0.033444 |
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Target: 5'- -gCGCCCCCaguaCUGCGGuUCCGGCAcCCg -3' miRNA: 3'- gaGCGGGGG----GACGCCuAGGCCGUaGG- -5' |
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| 12731 | 5' | -62 | NC_003356.1 | + | 28973 | 1.11 | 0.000123 |
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Target: 5'- uCUCGCCCCCCUGCGGAUCCGGCAUCCa -3' miRNA: 3'- -GAGCGGGGGGACGCCUAGGCCGUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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