Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12736 | 3' | -53.6 | NC_003356.1 | + | 29995 | 0.66 | 0.706214 |
Target: 5'- gCGCCGCUacccgcagUUCCGG-CuUCCGUCAc- -3' miRNA: 3'- gGUGGUGA--------AAGGCCuGuAGGCGGUaa -5' |
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12736 | 3' | -53.6 | NC_003356.1 | + | 37412 | 0.67 | 0.661216 |
Target: 5'- cCCGCCGauc-CCGGuuCGUCUGCCAc- -3' miRNA: 3'- -GGUGGUgaaaGGCCu-GUAGGCGGUaa -5' |
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12736 | 3' | -53.6 | NC_003356.1 | + | 26644 | 0.67 | 0.649865 |
Target: 5'- cCC-CCACccaUCCGGACGUugugCCGCCc-- -3' miRNA: 3'- -GGuGGUGaa-AGGCCUGUA----GGCGGuaa -5' |
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12736 | 3' | -53.6 | NC_003356.1 | + | 31962 | 0.68 | 0.627121 |
Target: 5'- gCCGCCACgauUUCgGcGAUAccuUCCGCCGa- -3' miRNA: 3'- -GGUGGUGa--AAGgC-CUGU---AGGCGGUaa -5' |
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12736 | 3' | -53.6 | NC_003356.1 | + | 33372 | 0.68 | 0.604396 |
Target: 5'- cCCAUCACca--CGuGGCAUCUGCCAUc -3' miRNA: 3'- -GGUGGUGaaagGC-CUGUAGGCGGUAa -5' |
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12736 | 3' | -53.6 | NC_003356.1 | + | 12891 | 0.72 | 0.365792 |
Target: 5'- uUCACCAa--UCUGGAUGUUCGCCAUUu -3' miRNA: 3'- -GGUGGUgaaAGGCCUGUAGGCGGUAA- -5' |
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12736 | 3' | -53.6 | NC_003356.1 | + | 32073 | 0.73 | 0.348234 |
Target: 5'- gCCAUCAuCUgaCCGGAUucuUCCGCCGUg -3' miRNA: 3'- -GGUGGU-GAaaGGCCUGu--AGGCGGUAa -5' |
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12736 | 3' | -53.6 | NC_003356.1 | + | 31857 | 1.07 | 0.001538 |
Target: 5'- uCCACCACUUUCCGGACAUCCGCCAUUu -3' miRNA: 3'- -GGUGGUGAAAGGCCUGUAGGCGGUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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