Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12745 | 3' | -56.2 | NC_003356.1 | + | 5716 | 0.66 | 0.537131 |
Target: 5'- cGAGCaGCAUGGCGaGuucGGCGGuuguuaagcCGCAGGCu -3' miRNA: 3'- -UUCG-CGUACCGC-C---UUGUCu--------GCGUCUG- -5' |
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12745 | 3' | -56.2 | NC_003356.1 | + | 13648 | 0.66 | 0.515247 |
Target: 5'- cAGCGCucaG-GGAACuGAUGCAGGCa -3' miRNA: 3'- uUCGCGuacCgCCUUGuCUGCGUCUG- -5' |
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12745 | 3' | -56.2 | NC_003356.1 | + | 31711 | 0.66 | 0.50444 |
Target: 5'- uGGGCGCAgcgGGUgccGGAACcgcaguacuggGGGCGCuGGCg -3' miRNA: 3'- -UUCGCGUa--CCG---CCUUG-----------UCUGCGuCUG- -5' |
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12745 | 3' | -56.2 | NC_003356.1 | + | 19544 | 0.67 | 0.472639 |
Target: 5'- --cCGCA-GGCGGGagaACAGAC-CGGACa -3' miRNA: 3'- uucGCGUaCCGCCU---UGUCUGcGUCUG- -5' |
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12745 | 3' | -56.2 | NC_003356.1 | + | 41876 | 0.67 | 0.462264 |
Target: 5'- uGAG-GCAUGGCGuacaGAACAGgaaGCGCAGcCg -3' miRNA: 3'- -UUCgCGUACCGC----CUUGUC---UGCGUCuG- -5' |
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12745 | 3' | -56.2 | NC_003356.1 | + | 36784 | 0.67 | 0.462264 |
Target: 5'- gAAGCGC-UGGCGuGGAUGGugGaGGAUg -3' miRNA: 3'- -UUCGCGuACCGC-CUUGUCugCgUCUG- -5' |
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12745 | 3' | -56.2 | NC_003356.1 | + | 38432 | 0.68 | 0.431882 |
Target: 5'- uGGCGUGUgacguugccGGUGGAugccggugcuCAGugGCAGACa -3' miRNA: 3'- uUCGCGUA---------CCGCCUu---------GUCugCGUCUG- -5' |
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12745 | 3' | -56.2 | NC_003356.1 | + | 34694 | 0.68 | 0.393252 |
Target: 5'- cAGGCGUAcGGCGGAugACAGGCuguuuuuucaGCuGACg -3' miRNA: 3'- -UUCGCGUaCCGCCU--UGUCUG----------CGuCUG- -5' |
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12745 | 3' | -56.2 | NC_003356.1 | + | 41176 | 0.7 | 0.291606 |
Target: 5'- gAAGCGCGUcGGUGGAuaacguuucauGguGAUGCAGAa -3' miRNA: 3'- -UUCGCGUA-CCGCCU-----------UguCUGCGUCUg -5' |
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12745 | 3' | -56.2 | NC_003356.1 | + | 40497 | 0.72 | 0.229723 |
Target: 5'- uGGCGCA-GGCGcAGCAGAgCGCGGAa -3' miRNA: 3'- uUCGCGUaCCGCcUUGUCU-GCGUCUg -5' |
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12745 | 3' | -56.2 | NC_003356.1 | + | 25120 | 0.72 | 0.229723 |
Target: 5'- -uGUGCaAUGGCacacGGAACAGACGaCAGAUu -3' miRNA: 3'- uuCGCG-UACCG----CCUUGUCUGC-GUCUG- -5' |
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12745 | 3' | -56.2 | NC_003356.1 | + | 21631 | 1.08 | 0.000538 |
Target: 5'- aAAGCGCAUGGCGGAACAGACGCAGACa -3' miRNA: 3'- -UUCGCGUACCGCCUUGUCUGCGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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