Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
12746 | 3' | -60.4 | NC_003356.1 | + | 24191 | 0.66 | 0.388682 |
Target: 5'- cGACCGuacaCGGCAGucuGUcGGCGGAGGu -3' miRNA: 3'- -CUGGUgcg-GUCGUCu--CGaCCGCCUCC- -5' |
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12746 | 3' | -60.4 | NC_003356.1 | + | 9591 | 0.66 | 0.387792 |
Target: 5'- cGCgCACGCCAgaauuauGguGGGCUgGGCaGGGGa -3' miRNA: 3'- cUG-GUGCGGU-------CguCUCGA-CCGcCUCC- -5' |
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12746 | 3' | -60.4 | NC_003356.1 | + | 16222 | 0.66 | 0.379839 |
Target: 5'- gGACCAgUGCCuugcGCAGuuCUGGCGGGu- -3' miRNA: 3'- -CUGGU-GCGGu---CGUCucGACCGCCUcc -5' |
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12746 | 3' | -60.4 | NC_003356.1 | + | 19687 | 0.66 | 0.379839 |
Target: 5'- cGCC-CGUUAGCAacGGGCUGGCuGGAa- -3' miRNA: 3'- cUGGuGCGGUCGU--CUCGACCG-CCUcc -5' |
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12746 | 3' | -60.4 | NC_003356.1 | + | 22734 | 0.66 | 0.365973 |
Target: 5'- aGACCaACGCCAGCAGAcauuGCUGauuucccguuuuuaCGGGGu -3' miRNA: 3'- -CUGG-UGCGGUCGUCU----CGACc-------------GCCUCc -5' |
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12746 | 3' | -60.4 | NC_003356.1 | + | 24340 | 0.66 | 0.35413 |
Target: 5'- aGCCucCGCCAGCucuGCUGGCGu-GGu -3' miRNA: 3'- cUGGu-GCGGUCGucuCGACCGCcuCC- -5' |
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12746 | 3' | -60.4 | NC_003356.1 | + | 25461 | 0.66 | 0.345837 |
Target: 5'- -gUCAuCGCCAGCAGAGggaCUGGUGGu-- -3' miRNA: 3'- cuGGU-GCGGUCGUCUC---GACCGCCucc -5' |
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12746 | 3' | -60.4 | NC_003356.1 | + | 35738 | 0.68 | 0.273393 |
Target: 5'- gGGCUGCGCUuacccgugAGacaacauauacgguuCAGGGCUGGCGGcAGGg -3' miRNA: 3'- -CUGGUGCGG--------UC---------------GUCUCGACCGCC-UCC- -5' |
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12746 | 3' | -60.4 | NC_003356.1 | + | 41346 | 0.71 | 0.173669 |
Target: 5'- cGACCAUGCUuuuGGUAGGuCUGGCGGAc- -3' miRNA: 3'- -CUGGUGCGG---UCGUCUcGACCGCCUcc -5' |
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12746 | 3' | -60.4 | NC_003356.1 | + | 24270 | 1.08 | 0.000229 |
Target: 5'- uGACCACGCCAGCAGAGCUGGCGGAGGc -3' miRNA: 3'- -CUGGUGCGGUCGUCUCGACCGCCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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