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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
12750 | 3' | -52.1 | NC_003356.1 | + | 31430 | 0.69 | 0.591768 |
Target: 5'- cGCCGCCGAUgaacGCGGGAuccccGCCAgcaGCGg- -3' miRNA: 3'- -CGGCGGCUA----CGUUUU-----CGGUaa-CGCaa -5' |
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12750 | 3' | -52.1 | NC_003356.1 | + | 40723 | 0.76 | 0.258393 |
Target: 5'- gGCCGCaucCGAUGCAGAgcaGGCCAgagaugauauugaugUUGCGUUa -3' miRNA: 3'- -CGGCG---GCUACGUUU---UCGGU---------------AACGCAA- -5' |
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12750 | 3' | -52.1 | NC_003356.1 | + | 27117 | 1.07 | 0.001675 |
Target: 5'- uGCCGCCGAUGCAAAAGCCAUUGCGUUu -3' miRNA: 3'- -CGGCGGCUACGUUUUCGGUAACGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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