Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1278 | 5' | -61 | NC_001317.1 | + | 18109 | 0.67 | 0.237533 |
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Target: 5'- -cCGcCGaugauaccaauCAGAGCUagaGcGCCACCGGCGAu -3' miRNA: 3'- uaGCaGC-----------GUCUCGA---C-CGGUGGCCGCU- -5' |
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| 1278 | 5' | -61 | NC_001317.1 | + | 7100 | 0.67 | 0.225254 |
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Target: 5'- ---aUCGCGGcGUUgaGGCCACCGGCa- -3' miRNA: 3'- uagcAGCGUCuCGA--CCGGUGGCCGcu -5' |
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| 1278 | 5' | -61 | NC_001317.1 | + | 9294 | 0.67 | 0.225254 |
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Target: 5'- -cCGcCGguGAcagGCUGGCaaucagACCGGCGAg -3' miRNA: 3'- uaGCaGCguCU---CGACCGg-----UGGCCGCU- -5' |
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| 1278 | 5' | -61 | NC_001317.1 | + | 30525 | 0.68 | 0.196903 |
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Target: 5'- -gCGUCGcCAGAGCUGGCg--CGGCu- -3' miRNA: 3'- uaGCAGC-GUCUCGACCGgugGCCGcu -5' |
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| 1278 | 5' | -61 | NC_001317.1 | + | 22074 | 0.68 | 0.176512 |
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Target: 5'- uAUCGccUCGCAcgcuuaaucuuGAGCUGGCCuacuuCCgGGCGGu -3' miRNA: 3'- -UAGC--AGCGU-----------CUCGACCGGu----GG-CCGCU- -5' |
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| 1278 | 5' | -61 | NC_001317.1 | + | 2741 | 1.06 | 0.00017 |
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Target: 5'- cAUCGUCGCAGAGCUGGCCACCGGCGAg -3' miRNA: 3'- -UAGCAGCGUCUCGACCGGUGGCCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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