Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12784 | 5' | -57.5 | NC_003387.1 | + | 31523 | 0.69 | 0.404465 |
Target: 5'- cUGuuCCACGCGGCCAgcacggcgugcaccUgCUCCUCgGAa -3' miRNA: 3'- aGCuuGGUGCGCCGGU--------------A-GAGGAGgCU- -5' |
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12784 | 5' | -57.5 | NC_003387.1 | + | 36791 | 0.69 | 0.398147 |
Target: 5'- gUCG-GCCGgGCGGCCuUCUCa-CCGAu -3' miRNA: 3'- -AGCuUGGUgCGCCGGuAGAGgaGGCU- -5' |
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12784 | 5' | -57.5 | NC_003387.1 | + | 1721 | 0.7 | 0.389231 |
Target: 5'- gCGGGCCGgGCGGCgCAUC-CCggugCUGAu -3' miRNA: 3'- aGCUUGGUgCGCCG-GUAGaGGa---GGCU- -5' |
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12784 | 5' | -57.5 | NC_003387.1 | + | 25438 | 0.7 | 0.389231 |
Target: 5'- -aGGGCCGCcaguGCGGCguUCUCCUCgCGc -3' miRNA: 3'- agCUUGGUG----CGCCGguAGAGGAG-GCu -5' |
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12784 | 5' | -57.5 | NC_003387.1 | + | 48797 | 0.7 | 0.363281 |
Target: 5'- cUGAucGCCGCggcgGCGGCCAUCgUCCcgCCGGu -3' miRNA: 3'- aGCU--UGGUG----CGCCGGUAG-AGGa-GGCU- -5' |
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12784 | 5' | -57.5 | NC_003387.1 | + | 14813 | 0.7 | 0.34666 |
Target: 5'- cCGAGCCACGCGGgCAgcgggUCgcCCUCgGGg -3' miRNA: 3'- aGCUUGGUGCGCCgGU-----AGa-GGAGgCU- -5' |
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12784 | 5' | -57.5 | NC_003387.1 | + | 7131 | 0.7 | 0.34666 |
Target: 5'- gUCGAucaGCCACGCgaGGCCcggCUCCUCgGc -3' miRNA: 3'- -AGCU---UGGUGCG--CCGGua-GAGGAGgCu -5' |
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12784 | 5' | -57.5 | NC_003387.1 | + | 29436 | 0.71 | 0.338556 |
Target: 5'- gCGGcCCGCGCGGUCGcuaUCCUCgCGAu -3' miRNA: 3'- aGCUuGGUGCGCCGGUag-AGGAG-GCU- -5' |
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12784 | 5' | -57.5 | NC_003387.1 | + | 4033 | 0.72 | 0.292847 |
Target: 5'- -aGAACCGCGUGGCgGcCUCCuggggcgacgauUCCGAg -3' miRNA: 3'- agCUUGGUGCGCCGgUaGAGG------------AGGCU- -5' |
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12784 | 5' | -57.5 | NC_003387.1 | + | 23302 | 0.75 | 0.175109 |
Target: 5'- gCGGGCCGCuGCGGCgGcCUCCUCCu- -3' miRNA: 3'- aGCUUGGUG-CGCCGgUaGAGGAGGcu -5' |
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12784 | 5' | -57.5 | NC_003387.1 | + | 47731 | 1.09 | 0.000662 |
Target: 5'- gUCGAACCACGCGGCCAUCUCCUCCGAc -3' miRNA: 3'- -AGCUUGGUGCGCCGGUAGAGGAGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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