Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12799 | 5' | -54.4 | NC_003387.1 | + | 19119 | 0.66 | 0.74976 |
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Target: 5'- gGUCGUGCcACA---CCUCGGCGg-GAu -3' miRNA: 3'- -CAGCACGuUGUagaGGAGCCGCagCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 34106 | 0.67 | 0.707143 |
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Target: 5'- uUCG-GCGGCAgaUCggCGGCGUCGAc -3' miRNA: 3'- cAGCaCGUUGUagAGgaGCCGCAGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 35507 | 0.67 | 0.685341 |
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Target: 5'- cGUCGaGCGGugUAUCUgCCUCGGCcUCGGc -3' miRNA: 3'- -CAGCaCGUU--GUAGA-GGAGCCGcAGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 22488 | 0.68 | 0.663332 |
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Target: 5'- cGUCGUaccggGCGACGg--CCUCGGCGgcgcgcUCGAc -3' miRNA: 3'- -CAGCA-----CGUUGUagaGGAGCCGC------AGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 25447 | 0.68 | 0.663332 |
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Target: 5'- --aGUGCGGCGuUCUCCUC-GCG-CGAc -3' miRNA: 3'- cagCACGUUGU-AGAGGAGcCGCaGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 4163 | 0.68 | 0.663332 |
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Target: 5'- cGUCGaUGCAcgccuggcACGUgaCCUCGGCG-CGGu -3' miRNA: 3'- -CAGC-ACGU--------UGUAgaGGAGCCGCaGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 8267 | 0.68 | 0.652278 |
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Target: 5'- -gCGUGCAGCGccUCgcgcagcgCCUCGugcGCGUCGGc -3' miRNA: 3'- caGCACGUUGU--AGa-------GGAGC---CGCAGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 8918 | 0.66 | 0.770388 |
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Target: 5'- aUCGUGCAGCA-CUCgUCGaggaaGCGggCGAc -3' miRNA: 3'- cAGCACGUUGUaGAGgAGC-----CGCa-GCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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