miRNA display CGI


Results 1 - 8 of 8 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1281 3' -59.1 NC_001317.1 + 2435 0.69 0.216976
Target:  5'- gCGGcGcGAgcacgacgcacCCGGCGCUGUCGCCAGUg -3'
miRNA:   3'- -GCCaCaCU-----------GGCCGUGGUAGCGGUCGu -5'
1281 3' -59.1 NC_001317.1 + 5029 1.08 0.000233
Target:  5'- uCGGUGUGACCGGCACCAUCGCCAGCAc -3'
miRNA:   3'- -GCCACACUGGCCGUGGUAGCGGUCGU- -5'
1281 3' -59.1 NC_001317.1 + 7571 0.67 0.320575
Target:  5'- gCGGgGUGAUgGGUucACCGccUGCCAGCAc -3'
miRNA:   3'- -GCCaCACUGgCCG--UGGUa-GCGGUCGU- -5'
1281 3' -59.1 NC_001317.1 + 8141 0.66 0.32866
Target:  5'- cCGGg--GGCCGGUuacgGCCAgCGCgAGCAc -3'
miRNA:   3'- -GCCacaCUGGCCG----UGGUaGCGgUCGU- -5'
1281 3' -59.1 NC_001317.1 + 8874 0.66 0.32866
Target:  5'- uCGGUGUGGCCguugucGGCAaaAagGCgCAGCAu -3'
miRNA:   3'- -GCCACACUGG------CCGUggUagCG-GUCGU- -5'
1281 3' -59.1 NC_001317.1 + 18043 0.67 0.289731
Target:  5'- cCGGUGgcgcucuagcucUGAUUGGUAUCAUCGgCGGUAu -3'
miRNA:   3'- -GCCAC------------ACUGGCCGUGGUAGCgGUCGU- -5'
1281 3' -59.1 NC_001317.1 + 20309 0.66 0.345279
Target:  5'- ----cUGAgCGGCACgCAUCGCCuGCGc -3'
miRNA:   3'- gccacACUgGCCGUG-GUAGCGGuCGU- -5'
1281 3' -59.1 NC_001317.1 + 24082 0.72 0.124437
Target:  5'- uCGGacUGUGAgCGGCACgGgUGCCAGCGc -3'
miRNA:   3'- -GCC--ACACUgGCCGUGgUaGCGGUCGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.