Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1281 | 3' | -59.1 | NC_001317.1 | + | 5029 | 1.08 | 0.000233 |
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Target: 5'- uCGGUGUGACCGGCACCAUCGCCAGCAc -3' miRNA: 3'- -GCCACACUGGCCGUGGUAGCGGUCGU- -5' |
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| 1281 | 3' | -59.1 | NC_001317.1 | + | 2435 | 0.69 | 0.216976 |
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Target: 5'- gCGGcGcGAgcacgacgcacCCGGCGCUGUCGCCAGUg -3' miRNA: 3'- -GCCaCaCU-----------GGCCGUGGUAGCGGUCGu -5' |
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| 1281 | 3' | -59.1 | NC_001317.1 | + | 18043 | 0.67 | 0.289731 |
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Target: 5'- cCGGUGgcgcucuagcucUGAUUGGUAUCAUCGgCGGUAu -3' miRNA: 3'- -GCCAC------------ACUGGCCGUGGUAGCgGUCGU- -5' |
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| 1281 | 3' | -59.1 | NC_001317.1 | + | 8141 | 0.66 | 0.32866 |
|
Target: 5'- cCGGg--GGCCGGUuacgGCCAgCGCgAGCAc -3' miRNA: 3'- -GCCacaCUGGCCG----UGGUaGCGgUCGU- -5' |
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| 1281 | 3' | -59.1 | NC_001317.1 | + | 24082 | 0.72 | 0.124437 |
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Target: 5'- uCGGacUGUGAgCGGCACgGgUGCCAGCGc -3' miRNA: 3'- -GCC--ACACUgGCCGUGgUaGCGGUCGU- -5' |
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| 1281 | 3' | -59.1 | NC_001317.1 | + | 7571 | 0.67 | 0.320575 |
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Target: 5'- gCGGgGUGAUgGGUucACCGccUGCCAGCAc -3' miRNA: 3'- -GCCaCACUGgCCG--UGGUa-GCGGUCGU- -5' |
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| 1281 | 3' | -59.1 | NC_001317.1 | + | 8874 | 0.66 | 0.32866 |
|
Target: 5'- uCGGUGUGGCCguugucGGCAaaAagGCgCAGCAu -3' miRNA: 3'- -GCCACACUGG------CCGUggUagCG-GUCGU- -5' |
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| 1281 | 3' | -59.1 | NC_001317.1 | + | 20309 | 0.66 | 0.345279 |
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Target: 5'- ----cUGAgCGGCACgCAUCGCCuGCGc -3' miRNA: 3'- gccacACUgGCCGUG-GUAGCGGuCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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