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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1282 | 5' | -55.8 | NC_001317.1 | + | 5612 | 1.06 | 0.000482 |
|
Target: 5'- cAUCAGCCAGAAGCGCAUCGGCAACCUg -3' miRNA: 3'- -UAGUCGGUCUUCGCGUAGCCGUUGGA- -5' |
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| 1282 | 5' | -55.8 | NC_001317.1 | + | 665 | 0.67 | 0.420182 |
|
Target: 5'- uUCAGCCAGGcggaauuaAGCGCGcugagauauUCGGgcaGGCCg -3' miRNA: 3'- uAGUCGGUCU--------UCGCGU---------AGCCg--UUGGa -5' |
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| 1282 | 5' | -55.8 | NC_001317.1 | + | 6943 | 0.67 | 0.410203 |
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Target: 5'- ---uGCgCAGgcGCGCAUCGGaAACCg -3' miRNA: 3'- uaguCG-GUCuuCGCGUAGCCgUUGGa -5' |
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| 1282 | 5' | -55.8 | NC_001317.1 | + | 16830 | 0.66 | 0.461515 |
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Target: 5'- cAUCAGCUcGGAGCGC--UGGCGcuguACCg -3' miRNA: 3'- -UAGUCGGuCUUCGCGuaGCCGU----UGGa -5' |
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| 1282 | 5' | -55.8 | NC_001317.1 | + | 19899 | 0.66 | 0.472181 |
|
Target: 5'- gGUCAGCCAGucgGGUcagaaauacgGCGUCGGacuCCUg -3' miRNA: 3'- -UAGUCGGUCu--UCG----------CGUAGCCguuGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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