Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1284 | 3' | -54.9 | NC_001317.1 | + | 6239 | 1.07 | 0.000685 |
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Target: 5'- uCGCCGCACGACACCUUACAUGCUGGUc -3' miRNA: 3'- -GCGGCGUGCUGUGGAAUGUACGACCA- -5' |
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| 1284 | 3' | -54.9 | NC_001317.1 | + | 6281 | 0.71 | 0.301608 |
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Target: 5'- uGCCGCGCaGCGCCUgcGCgAUGCcGGUg -3' miRNA: 3'- gCGGCGUGcUGUGGAa-UG-UACGaCCA- -5' |
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| 1284 | 3' | -54.9 | NC_001317.1 | + | 805 | 0.67 | 0.479363 |
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Target: 5'- uGCCGCAUGaauucGCGCCUgAUAccguguuucaccUGCUGGa -3' miRNA: 3'- gCGGCGUGC-----UGUGGAaUGU------------ACGACCa -5' |
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| 1284 | 3' | -54.9 | NC_001317.1 | + | 27371 | 0.67 | 0.511942 |
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Target: 5'- gGCCGCuuGCGACGCaUUGCGgcUGCaUGGc -3' miRNA: 3'- gCGGCG--UGCUGUGgAAUGU--ACG-ACCa -5' |
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| 1284 | 3' | -54.9 | NC_001317.1 | + | 10932 | 0.66 | 0.523006 |
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Target: 5'- aGCUGCuCG-CGCag-GCAUGCUGGc -3' miRNA: 3'- gCGGCGuGCuGUGgaaUGUACGACCa -5' |
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| 1284 | 3' | -54.9 | NC_001317.1 | + | 18118 | 0.66 | 0.556712 |
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Target: 5'- aCGCCauuauCGCuGCugCUgGCGUGCUGGg -3' miRNA: 3'- -GCGGc----GUGcUGugGAaUGUACGACCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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