miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1284 3' -54.9 NC_001317.1 + 805 0.67 0.479363
Target:  5'- uGCCGCAUGaauucGCGCCUgAUAccguguuucaccUGCUGGa -3'
miRNA:   3'- gCGGCGUGC-----UGUGGAaUGU------------ACGACCa -5'
1284 3' -54.9 NC_001317.1 + 6239 1.07 0.000685
Target:  5'- uCGCCGCACGACACCUUACAUGCUGGUc -3'
miRNA:   3'- -GCGGCGUGCUGUGGAAUGUACGACCA- -5'
1284 3' -54.9 NC_001317.1 + 6281 0.71 0.301608
Target:  5'- uGCCGCGCaGCGCCUgcGCgAUGCcGGUg -3'
miRNA:   3'- gCGGCGUGcUGUGGAa-UG-UACGaCCA- -5'
1284 3' -54.9 NC_001317.1 + 10932 0.66 0.523006
Target:  5'- aGCUGCuCG-CGCag-GCAUGCUGGc -3'
miRNA:   3'- gCGGCGuGCuGUGgaaUGUACGACCa -5'
1284 3' -54.9 NC_001317.1 + 18118 0.66 0.556712
Target:  5'- aCGCCauuauCGCuGCugCUgGCGUGCUGGg -3'
miRNA:   3'- -GCGGc----GUGcUGugGAaUGUACGACCa -5'
1284 3' -54.9 NC_001317.1 + 27371 0.67 0.511942
Target:  5'- gGCCGCuuGCGACGCaUUGCGgcUGCaUGGc -3'
miRNA:   3'- gCGGCG--UGCUGUGgAAUGU--ACG-ACCa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.