Results 41 - 48 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12994 | 3' | -54.2 | NC_003387.1 | + | 50451 | 0.72 | 0.420629 |
Target: 5'- gCGcCCUCGGgcaCCUCGGG-GCGccCACGCa -3' miRNA: 3'- -GC-GGAGUUa--GGAGCCCuUGCa-GUGCG- -5' |
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12994 | 3' | -54.2 | NC_003387.1 | + | 50573 | 0.73 | 0.392863 |
Target: 5'- gCGgCUCAucgaGUCCUCGGGGaacuccACGUCGgcCGCu -3' miRNA: 3'- -GCgGAGU----UAGGAGCCCU------UGCAGU--GCG- -5' |
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12994 | 3' | -54.2 | NC_003387.1 | + | 17102 | 0.74 | 0.357744 |
Target: 5'- cCGCCUCGGcgggcUCCUCGGGGcCGUaggcgauCGCu -3' miRNA: 3'- -GCGGAGUU-----AGGAGCCCUuGCAgu-----GCG- -5' |
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12994 | 3' | -54.2 | NC_003387.1 | + | 49694 | 0.74 | 0.356895 |
Target: 5'- gCGCCUUca-CCUCGGGAucuucaaACGUCcacACGCg -3' miRNA: 3'- -GCGGAGuuaGGAGCCCU-------UGCAG---UGCG- -5' |
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12994 | 3' | -54.2 | NC_003387.1 | + | 34519 | 0.75 | 0.294326 |
Target: 5'- gCGCCgCGAUCCUCGGGcugccCGUCGaccCGCu -3' miRNA: 3'- -GCGGaGUUAGGAGCCCuu---GCAGU---GCG- -5' |
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12994 | 3' | -54.2 | NC_003387.1 | + | 23032 | 0.76 | 0.259338 |
Target: 5'- gCGCCUCGA-CCUCGGccACGUCgACGUc -3' miRNA: 3'- -GCGGAGUUaGGAGCCcuUGCAG-UGCG- -5' |
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12994 | 3' | -54.2 | NC_003387.1 | + | 12169 | 0.84 | 0.084374 |
Target: 5'- gCGCCUCGAacgCCUCGGGG--GUCACGCc -3' miRNA: 3'- -GCGGAGUUa--GGAGCCCUugCAGUGCG- -5' |
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12994 | 3' | -54.2 | NC_003387.1 | + | 48741 | 1.13 | 0.00076 |
Target: 5'- cCGCCUCAAUCCUCGGGAACGUCACGCa -3' miRNA: 3'- -GCGGAGUUAGGAGCCCUUGCAGUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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