Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13005 | 5' | -54.4 | NC_003387.1 | + | 49590 | 0.67 | 0.716115 |
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Target: 5'- gCCgGUGACcuGAGCCGGGCUGaucugCGCGa-- -3' miRNA: 3'- -GG-CGCUG--CUCGGUCUGACa----GUGCaaa -5' |
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| 13005 | 5' | -54.4 | NC_003387.1 | + | 17028 | 0.66 | 0.726848 |
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Target: 5'- aCCGCGGCGGcugccgcccucGCCGGGC-GUCGuCGa-- -3' miRNA: 3'- -GGCGCUGCU-----------CGGUCUGaCAGU-GCaaa -5' |
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| 13005 | 5' | -54.4 | NC_003387.1 | + | 47756 | 0.66 | 0.752191 |
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Target: 5'- gCUGCG-CGAGCaCGGccugaccggcugguCUGUCACGUUc -3' miRNA: 3'- -GGCGCuGCUCG-GUCu-------------GACAGUGCAAa -5' |
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| 13005 | 5' | -54.4 | NC_003387.1 | + | 20474 | 0.66 | 0.758421 |
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Target: 5'- uCgGCGACG-GCCAGGCgGUCGg---- -3' miRNA: 3'- -GgCGCUGCuCGGUCUGaCAGUgcaaa -5' |
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| 13005 | 5' | -54.4 | NC_003387.1 | + | 12988 | 0.66 | 0.768699 |
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Target: 5'- aCCGaCGACGAGCUuaAGGC-GUCGuCGg-- -3' miRNA: 3'- -GGC-GCUGCUCGG--UCUGaCAGU-GCaaa -5' |
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| 13005 | 5' | -54.4 | NC_003387.1 | + | 45836 | 0.66 | 0.768699 |
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Target: 5'- cCCGCGACGAcGCCgcggAGAucCUG-CGCGa-- -3' miRNA: 3'- -GGCGCUGCU-CGG----UCU--GACaGUGCaaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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