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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 1306 | 5' | -61.2 | NC_001331.1 | + | 595 | 0.67 | 0.063494 |
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Target: 5'- gACCUcgaCCCCCGAGGgcuUCAUG-GCGuGUCg -3' miRNA: 3'- -UGGA---GGGGGCUCC---GGUAUgCGCuCGG- -5' |
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| 1306 | 5' | -61.2 | NC_001331.1 | + | 650 | 0.67 | 0.065581 |
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Target: 5'- uACCugauUCCgCCCGAGGCCGcuggaUACGUGgacauccuGGUCa -3' miRNA: 3'- -UGG----AGG-GGGCUCCGGU-----AUGCGC--------UCGG- -5' |
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| 1306 | 5' | -61.2 | NC_001331.1 | + | 2116 | 0.67 | 0.055586 |
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Target: 5'- cCCUCCCCCGcuGCCGUcaccauuGC-CGGuGCCg -3' miRNA: 3'- uGGAGGGGGCucCGGUA-------UGcGCU-CGG- -5' |
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| 1306 | 5' | -61.2 | NC_001331.1 | + | 3277 | 0.69 | 0.040234 |
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Target: 5'- aGCCagCCCaUGGGGCgGUcggccGCGUGGGCCg -3' miRNA: 3'- -UGGagGGG-GCUCCGgUA-----UGCGCUCGG- -5' |
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| 1306 | 5' | -61.2 | NC_001331.1 | + | 5272 | 1.1 | 0.000009 |
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Target: 5'- gACCUCCCCCGAGGCCAUACGCGAGCCu -3' miRNA: 3'- -UGGAGGGGGCUCCGGUAUGCGCUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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