miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1306 5' -61.2 NC_001331.1 + 5272 1.1 0.000009
Target:  5'- gACCUCCCCCGAGGCCAUACGCGAGCCu -3'
miRNA:   3'- -UGGAGGGGGCUCCGGUAUGCGCUCGG- -5'
1306 5' -61.2 NC_001331.1 + 3277 0.69 0.040234
Target:  5'- aGCCagCCCaUGGGGCgGUcggccGCGUGGGCCg -3'
miRNA:   3'- -UGGagGGG-GCUCCGgUA-----UGCGCUCGG- -5'
1306 5' -61.2 NC_001331.1 + 2116 0.67 0.055586
Target:  5'- cCCUCCCCCGcuGCCGUcaccauuGC-CGGuGCCg -3'
miRNA:   3'- uGGAGGGGGCucCGGUA-------UGcGCU-CGG- -5'
1306 5' -61.2 NC_001331.1 + 595 0.67 0.063494
Target:  5'- gACCUcgaCCCCCGAGGgcuUCAUG-GCGuGUCg -3'
miRNA:   3'- -UGGA---GGGGGCUCC---GGUAUgCGCuCGG- -5'
1306 5' -61.2 NC_001331.1 + 650 0.67 0.065581
Target:  5'- uACCugauUCCgCCCGAGGCCGcuggaUACGUGgacauccuGGUCa -3'
miRNA:   3'- -UGG----AGG-GGGCUCCGGU-----AUGCGC--------UCGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.