Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13091 | 3' | -60.2 | NC_003401.1 | + | 132517 | 0.73 | 0.293811 |
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Target: 5'- gGGCGGccGgGGGC-GGCGGCCGcggGCCCg -3' miRNA: 3'- gUCGCU--CgUCCGuCCGUCGGCa--UGGG- -5' |
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| 13091 | 3' | -60.2 | NC_003401.1 | + | 121925 | 0.73 | 0.278592 |
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Target: 5'- gAGCGGGgGGcGCGGGUgcggcggcuggcgcGGCCGUGCCa -3' miRNA: 3'- gUCGCUCgUC-CGUCCG--------------UCGGCAUGGg -5' |
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| 13091 | 3' | -60.2 | NC_003401.1 | + | 57741 | 0.74 | 0.276654 |
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Target: 5'- gCGGCGAGCGGGUauaccuGGGCGGCCauugauaaaacaacaGUAUUCa -3' miRNA: 3'- -GUCGCUCGUCCG------UCCGUCGG---------------CAUGGG- -5' |
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| 13091 | 3' | -60.2 | NC_003401.1 | + | 116524 | 0.74 | 0.243592 |
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Target: 5'- cCAG-GAGCcuuGGCAGGCAGUCGcACCUc -3' miRNA: 3'- -GUCgCUCGu--CCGUCCGUCGGCaUGGG- -5' |
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| 13091 | 3' | -60.2 | NC_003401.1 | + | 132957 | 1.11 | 0.000623 |
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Target: 5'- cCAGCGAGCAGGCAGGCAGCCGUACCCg -3' miRNA: 3'- -GUCGCUCGUCCGUCCGUCGGCAUGGG- -5' |
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| 13091 | 3' | -60.2 | NC_003401.1 | + | 222 | 1.11 | 0.000623 |
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Target: 5'- cCAGCGAGCAGGCAGGCAGCCGUACCCg -3' miRNA: 3'- -GUCGCUCGUCCGUCCGUCGGCAUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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