Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13091 | 5' | -58.4 | NC_003401.1 | + | 133071 | 0.66 | 0.78809 |
Target: 5'- aGCAAGCAgGCcagcgagcaaGCAgGCCuacuGUCCCCUc- -3' miRNA: 3'- -UGUUCGUgCG----------UGUgCGG----CAGGGGAca -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 133007 | 0.66 | 0.78809 |
Target: 5'- aGCAAGCAgGCcagcgagcaaGCAgGCCuacuGUCCCCUc- -3' miRNA: 3'- -UGUUCGUgCG----------UGUgCGG----CAGGGGAca -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 272 | 0.66 | 0.78809 |
Target: 5'- aGCAAGCAgGCcagcgagcaaGCAgGCCuacuGUCCCCUc- -3' miRNA: 3'- -UGUUCGUgCG----------UGUgCGG----CAGGGGAca -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 336 | 0.66 | 0.78809 |
Target: 5'- aGCAAGCAgGCcagcgagcaaGCAgGCCuacuGUCCCCUc- -3' miRNA: 3'- -UGUUCGUgCG----------UGUgCGG----CAGGGGAca -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 109934 | 0.66 | 0.778885 |
Target: 5'- uGCAGGCGCacgguGCACACGgggaCCG-CCCCg-- -3' miRNA: 3'- -UGUUCGUG-----CGUGUGC----GGCaGGGGaca -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 80695 | 0.66 | 0.778885 |
Target: 5'- cCGGGuCAUGCgAUugGCuCGUCCCCUc- -3' miRNA: 3'- uGUUC-GUGCG-UGugCG-GCAGGGGAca -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 20613 | 0.66 | 0.778885 |
Target: 5'- gGCcAGCccuACGCugGCGCC-UCCCCcGa -3' miRNA: 3'- -UGuUCG---UGCGugUGCGGcAGGGGaCa -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 64694 | 0.66 | 0.778885 |
Target: 5'- -aAGGCGCGC-CAU-CCGucUCCCCUGg -3' miRNA: 3'- ugUUCGUGCGuGUGcGGC--AGGGGACa -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 122361 | 0.66 | 0.769548 |
Target: 5'- gUAAGuCACGCGCGcCGCgGcCCCCUu- -3' miRNA: 3'- uGUUC-GUGCGUGU-GCGgCaGGGGAca -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 108837 | 0.66 | 0.769548 |
Target: 5'- uGCGAGaaCugGCGC-CGaCCGUCCCgUGc -3' miRNA: 3'- -UGUUC--GugCGUGuGC-GGCAGGGgACa -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 27703 | 0.66 | 0.73792 |
Target: 5'- cGCGGGCcccgguggaggccaGCGCACA-GCCGgCCCCcGUg -3' miRNA: 3'- -UGUUCG--------------UGCGUGUgCGGCaGGGGaCA- -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 44878 | 0.67 | 0.711283 |
Target: 5'- --cAGCGCGCACACuauggGCUGg-CCCUGUu -3' miRNA: 3'- uguUCGUGCGUGUG-----CGGCagGGGACA- -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 8913 | 0.67 | 0.695255 |
Target: 5'- cGCAGGCGCccuGUggcggcgaccggcugACGCGCCGUCgUCUGg -3' miRNA: 3'- -UGUUCGUG---CG---------------UGUGCGGCAGgGGACa -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 14513 | 0.68 | 0.660795 |
Target: 5'- gACGGGCuuGCggucGCGCGCCGUCCacaUGg -3' miRNA: 3'- -UGUUCGugCG----UGUGCGGCAGGgg-ACa -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 121941 | 0.68 | 0.659775 |
Target: 5'- uGCGgcGGCugGCGCG-GCCGUgccacgcCCCCUGc -3' miRNA: 3'- -UGU--UCGugCGUGUgCGGCA-------GGGGACa -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 23314 | 0.68 | 0.650593 |
Target: 5'- cACAAGCACGCccccgcccuGCAC-CCGgcUCCCCgagGUc -3' miRNA: 3'- -UGUUCGUGCG---------UGUGcGGC--AGGGGa--CA- -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 14693 | 0.69 | 0.619934 |
Target: 5'- cACGuuCACGCACACgcugguGCCGUCCUCgGUu -3' miRNA: 3'- -UGUucGUGCGUGUG------CGGCAGGGGaCA- -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 52572 | 0.69 | 0.599528 |
Target: 5'- --uAGUACGCGCcUGCCGggagaaacaUCCCCUGa -3' miRNA: 3'- uguUCGUGCGUGuGCGGC---------AGGGGACa -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 14870 | 0.69 | 0.599528 |
Target: 5'- uACucGUGCGCGCGCGCCacgCCCCg-- -3' miRNA: 3'- -UGuuCGUGCGUGUGCGGca-GGGGaca -5' |
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13091 | 5' | -58.4 | NC_003401.1 | + | 71622 | 0.69 | 0.589357 |
Target: 5'- cCAGGUugGgCACAcCGaCCGUCCCCg-- -3' miRNA: 3'- uGUUCGugC-GUGU-GC-GGCAGGGGaca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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