Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13097 | 3' | -61.9 | NC_003401.1 | + | 22302 | 0.66 | 0.654145 |
Target: 5'- -aACaCCCUCGacguagACGCAGCUGGgCGa -3' miRNA: 3'- ccUGcGGGAGCg-----UGCGUCGACCgGCc -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 132508 | 0.66 | 0.654145 |
Target: 5'- gGGGCggcggccgcggGCCCggGCcgGCGgGGCggagGGCCGGg -3' miRNA: 3'- -CCUG-----------CGGGagCG--UGCgUCGa---CCGGCC- -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 17500 | 0.66 | 0.654145 |
Target: 5'- cGGACGCgg-CGCGCGUGGCcc-CCGGg -3' miRNA: 3'- -CCUGCGggaGCGUGCGUCGaccGGCC- -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 9358 | 0.66 | 0.653163 |
Target: 5'- cGAC-CCCUaGCGC-CGGCgcccuacUGGCCGGg -3' miRNA: 3'- cCUGcGGGAgCGUGcGUCG-------ACCGGCC- -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 132721 | 0.66 | 0.648252 |
Target: 5'- cGGcCGCCCggcaucgggagccgCGCGcCGCGGCUuguggggccccgGGcCCGGg -3' miRNA: 3'- -CCuGCGGGa-------------GCGU-GCGUCGA------------CC-GGCC- -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 12929 | 0.66 | 0.64432 |
Target: 5'- cGACGCUucUUCGaACGCGGUUGGCgGu -3' miRNA: 3'- cCUGCGG--GAGCgUGCGUCGACCGgCc -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 132642 | 0.66 | 0.64432 |
Target: 5'- aGGugGUCUcCGCGcCGCcgggaGGggGGCCGGg -3' miRNA: 3'- -CCugCGGGaGCGU-GCG-----UCgaCCGGCC- -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 14915 | 0.66 | 0.64432 |
Target: 5'- aGGACgGCCCUgGaguuugACuGCAGCUGGgaGGa -3' miRNA: 3'- -CCUG-CGGGAgCg-----UG-CGUCGACCggCC- -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 104880 | 0.66 | 0.641371 |
Target: 5'- gGGGCGCgCUgccgCGCACGUgaaggugauuggucAGuCUGGCCa- -3' miRNA: 3'- -CCUGCG-GGa---GCGUGCG--------------UC-GACCGGcc -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 83405 | 0.66 | 0.639404 |
Target: 5'- uGGACaagguuuggcauuGCCg-UGCACGCGGCgaggauauggagcGGCCGGu -3' miRNA: 3'- -CCUG-------------CGGgaGCGUGCGUCGa------------CCGGCC- -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 75644 | 0.66 | 0.633503 |
Target: 5'- cGAC-CCC-CGCAacgguuaacccucCGCAGCUGuGUCGGc -3' miRNA: 3'- cCUGcGGGaGCGU-------------GCGUCGAC-CGGCC- -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 116644 | 0.66 | 0.62465 |
Target: 5'- aGGGCGgUUggaCGCugGCGcGUUGGCCGu -3' miRNA: 3'- -CCUGCgGGa--GCGugCGU-CGACCGGCc -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 72459 | 0.66 | 0.62465 |
Target: 5'- aGGGCGUCUUccaucugccCGCGCGUcacGGCcuUGGCCGu -3' miRNA: 3'- -CCUGCGGGA---------GCGUGCG---UCG--ACCGGCc -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 20540 | 0.66 | 0.62465 |
Target: 5'- --uCGCUUacaaUCGCGgGCGGC-GGCCGGu -3' miRNA: 3'- ccuGCGGG----AGCGUgCGUCGaCCGGCC- -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 132448 | 0.66 | 0.61482 |
Target: 5'- cGGCGCCCg-GCGCGCg---GGcCCGGg -3' miRNA: 3'- cCUGCGGGagCGUGCGucgaCC-GGCC- -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 132857 | 0.67 | 0.595202 |
Target: 5'- cGGgGCCCUCGguCGC-GCcccGcGCCGGg -3' miRNA: 3'- cCUgCGGGAGCguGCGuCGa--C-CGGCC- -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 20759 | 0.67 | 0.575684 |
Target: 5'- aGGGCgcgGCCCcgCGUugGgGGUUggcGGCCGGu -3' miRNA: 3'- -CCUG---CGGGa-GCGugCgUCGA---CCGGCC- -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 119353 | 0.67 | 0.565977 |
Target: 5'- cGGugGCUCUCGCAaauggaaaagGCGGCccUGGUCa- -3' miRNA: 3'- -CCugCGGGAGCGUg---------CGUCG--ACCGGcc -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 40343 | 0.67 | 0.564041 |
Target: 5'- aGGACuCCCUCGCcaaaacaccgauCGCGGC-GGCUGu -3' miRNA: 3'- -CCUGcGGGAGCGu-----------GCGUCGaCCGGCc -5' |
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13097 | 3' | -61.9 | NC_003401.1 | + | 126057 | 0.67 | 0.556314 |
Target: 5'- uGGCGCaCCUCGUcgACGU-GCUggGGCUGGa -3' miRNA: 3'- cCUGCG-GGAGCG--UGCGuCGA--CCGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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