Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13102 | 3' | -62.7 | NC_003401.1 | + | 132534 | 0.66 | 0.627662 |
Target: 5'- gCCCGgCgcgcgGCgCggGGCGgcCGGGGGCg -3' miRNA: 3'- -GGGCgGa----CGgGgaCCGCaaGCCUCCG- -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 132655 | 0.66 | 0.609076 |
Target: 5'- gCCGCCgggaggggggccgggGCCCggGGCGccUCGGcgGGGCc -3' miRNA: 3'- gGGCGGa--------------CGGGgaCCGCa-AGCC--UCCG- -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 132444 | 0.66 | 0.608099 |
Target: 5'- gCCCGgCgcgcggGCCCggggGGCGcgCGGGGGa -3' miRNA: 3'- -GGGCgGa-----CGGGga--CCGCaaGCCUCCg -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 91633 | 0.66 | 0.605169 |
Target: 5'- -aCGCaCUGCUCUUuuaaguccaggucaGGCGUucUCGGAGGg -3' miRNA: 3'- ggGCG-GACGGGGA--------------CCGCA--AGCCUCCg -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 14233 | 0.66 | 0.598339 |
Target: 5'- cCCCGCCUGCCuCCgaacGCcaggGGcAGGCg -3' miRNA: 3'- -GGGCGGACGG-GGac--CGcaagCC-UCCG- -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 132499 | 0.66 | 0.598339 |
Target: 5'- gCCGCggGCCCgggccGGCGgggCGGAGGg -3' miRNA: 3'- gGGCGgaCGGGga---CCGCaa-GCCUCCg -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 106913 | 0.66 | 0.598339 |
Target: 5'- aCCGUgguggUUGCCUCUGGUuggCGaGAGGCu -3' miRNA: 3'- gGGCG-----GACGGGGACCGcaaGC-CUCCG- -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 89666 | 0.66 | 0.588601 |
Target: 5'- aCCGUCaGCCuCCgccgGaGCGggaGGAGGCa -3' miRNA: 3'- gGGCGGaCGG-GGa---C-CGCaagCCUCCG- -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 132828 | 0.66 | 0.584714 |
Target: 5'- cCCCGCCgaggcgccccggGCCCCggccccccucccGGCGgcgCGGAGa- -3' miRNA: 3'- -GGGCGGa-----------CGGGGa-----------CCGCaa-GCCUCcg -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 127721 | 0.66 | 0.578893 |
Target: 5'- aCCCGCCUcugucaucgGCCaCCUGGgagcccUGgucUCGcGGGGCg -3' miRNA: 3'- -GGGCGGA---------CGG-GGACC------GCa--AGC-CUCCG- -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 73 | 0.66 | 0.569219 |
Target: 5'- gCCUGCCUGCUCgCUGGCcugcuugCuGAGGg -3' miRNA: 3'- -GGGCGGACGGG-GACCGcaa----GcCUCCg -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 132808 | 0.66 | 0.569219 |
Target: 5'- gCCUGCCUGCUCgCUGGCcugcuugCuGAGGg -3' miRNA: 3'- -GGGCGGACGGG-GACCGcaa----GcCUCCg -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 68073 | 0.66 | 0.569219 |
Target: 5'- aCUCaGUaCUGCCCCUGGUGgaUGcGGGCu -3' miRNA: 3'- -GGG-CG-GACGGGGACCGCaaGCcUCCG- -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 48499 | 0.67 | 0.553828 |
Target: 5'- aCgCGCCUGCgCCCacgucuuuggauaguUGGCuuGUUUGGgaGGGCg -3' miRNA: 3'- -GgGCGGACG-GGG---------------ACCG--CAAGCC--UCCG- -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 91114 | 0.67 | 0.544271 |
Target: 5'- cCCCGguguuuuugCUGCCCCUGGUGgccuuuauaguaGGuGGCg -3' miRNA: 3'- -GGGCg--------GACGGGGACCGCaag---------CCuCCG- -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 119493 | 0.68 | 0.502929 |
Target: 5'- gCCCGUUUGCaaacCCCUcgGGCGUaucgUCGGAcgccGGCc -3' miRNA: 3'- -GGGCGGACG----GGGA--CCGCA----AGCCU----CCG- -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 17707 | 0.68 | 0.457699 |
Target: 5'- gCCUGCCcggugGaCCCCUGcGCGUcucCGGcgAGGCg -3' miRNA: 3'- -GGGCGGa----C-GGGGAC-CGCAa--GCC--UCCG- -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 29564 | 0.69 | 0.443687 |
Target: 5'- gUCGCCUGCUuguggcacugagcuaCUguuUGGCGUUuUGGGGGCa -3' miRNA: 3'- gGGCGGACGG---------------GG---ACCGCAA-GCCUCCG- -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 18022 | 0.69 | 0.431628 |
Target: 5'- aCCCGuCCUGCCCacccacaccGGCGgccgcguaUUGGGGGUc -3' miRNA: 3'- -GGGC-GGACGGGga-------CCGCa-------AGCCUCCG- -5' |
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13102 | 3' | -62.7 | NC_003401.1 | + | 15937 | 0.69 | 0.423132 |
Target: 5'- gCCCcCCUGUCCCUcgGGCGUUggaaccgGGAGGa -3' miRNA: 3'- -GGGcGGACGGGGA--CCGCAAg------CCUCCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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