Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13102 | 3' | -62.7 | NC_003401.1 | + | 17325 | 0.69 | 0.406446 |
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Target: 5'- aCCCGCCUGCaaaCguucaagGGCca-CGGGGGCu -3' miRNA: 3'- -GGGCGGACGgg-Ga------CCGcaaGCCUCCG- -5' |
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| 13102 | 3' | -62.7 | NC_003401.1 | + | 19804 | 0.69 | 0.406446 |
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Target: 5'- cCCCGCCcggcaGCUCCagGGCGcgcgaUCGGuGGCc -3' miRNA: 3'- -GGGCGGa----CGGGGa-CCGCa----AGCCuCCG- -5' |
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| 13102 | 3' | -62.7 | NC_003401.1 | + | 75098 | 0.7 | 0.398259 |
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Target: 5'- gCCCGCC-GCCUg-GGCGcUUGGcGGGCa -3' miRNA: 3'- -GGGCGGaCGGGgaCCGCaAGCC-UCCG- -5' |
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| 13102 | 3' | -62.7 | NC_003401.1 | + | 118843 | 0.75 | 0.181369 |
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Target: 5'- uCCgCGCCagcgGCCCCUcGCGUUgCGGGGGUc -3' miRNA: 3'- -GG-GCGGa---CGGGGAcCGCAA-GCCUCCG- -5' |
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| 13102 | 3' | -62.7 | NC_003401.1 | + | 23442 | 0.77 | 0.135336 |
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Target: 5'- cCCCGCC-GCgCCgGGuCGUUCGGAGGg -3' miRNA: 3'- -GGGCGGaCGgGGaCC-GCAAGCCUCCg -5' |
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| 13102 | 3' | -62.7 | NC_003401.1 | + | 14400 | 1.12 | 0.000406 |
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Target: 5'- uCCCGCCUGCCCCUGGCGUUCGGAGGCa -3' miRNA: 3'- -GGGCGGACGGGGACCGCAAGCCUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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