Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13102 | 3' | -62.7 | NC_003401.1 | + | 132808 | 0.66 | 0.569219 |
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Target: 5'- gCCUGCCUGCUCgCUGGCcugcuugCuGAGGg -3' miRNA: 3'- -GGGCGGACGGG-GACCGcaa----GcCUCCg -5' |
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| 13102 | 3' | -62.7 | NC_003401.1 | + | 89666 | 0.66 | 0.588601 |
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Target: 5'- aCCGUCaGCCuCCgccgGaGCGggaGGAGGCa -3' miRNA: 3'- gGGCGGaCGG-GGa---C-CGCaagCCUCCG- -5' |
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| 13102 | 3' | -62.7 | NC_003401.1 | + | 14233 | 0.66 | 0.598339 |
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Target: 5'- cCCCGCCUGCCuCCgaacGCcaggGGcAGGCg -3' miRNA: 3'- -GGGCGGACGG-GGac--CGcaagCC-UCCG- -5' |
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| 13102 | 3' | -62.7 | NC_003401.1 | + | 91633 | 0.66 | 0.605169 |
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Target: 5'- -aCGCaCUGCUCUUuuaaguccaggucaGGCGUucUCGGAGGg -3' miRNA: 3'- ggGCG-GACGGGGA--------------CCGCA--AGCCUCCg -5' |
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| 13102 | 3' | -62.7 | NC_003401.1 | + | 132655 | 0.66 | 0.609076 |
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Target: 5'- gCCGCCgggaggggggccgggGCCCggGGCGccUCGGcgGGGCc -3' miRNA: 3'- gGGCGGa--------------CGGGgaCCGCa-AGCC--UCCG- -5' |
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| 13102 | 3' | -62.7 | NC_003401.1 | + | 23442 | 0.77 | 0.135336 |
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Target: 5'- cCCCGCC-GCgCCgGGuCGUUCGGAGGg -3' miRNA: 3'- -GGGCGGaCGgGGaCC-GCAAGCCUCCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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