Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13111 | 5' | -46.3 | NC_003401.1 | + | 28338 | 0.66 | 0.999866 |
Target: 5'- cGCCGccuGAGCUGACCG-UCCcAUCGUa -3' miRNA: 3'- -UGGUuu-UUUGAUUGGUgAGGaUGGCA- -5' |
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13111 | 5' | -46.3 | NC_003401.1 | + | 72720 | 0.66 | 0.999866 |
Target: 5'- cACCGAAAAGgaGGCCAaaaUCgcagCUGCCGUu -3' miRNA: 3'- -UGGUUUUUUgaUUGGUg--AG----GAUGGCA- -5' |
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13111 | 5' | -46.3 | NC_003401.1 | + | 45150 | 0.66 | 0.999866 |
Target: 5'- uGCCGAuuuAAAAUccGACCACUCCguCCGg -3' miRNA: 3'- -UGGUU---UUUUGa-UUGGUGAGGauGGCa -5' |
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13111 | 5' | -46.3 | NC_003401.1 | + | 88049 | 0.66 | 0.999898 |
Target: 5'- cACCuAGGAGGCguucCCACUCCggaaggcgaGCCGUg -3' miRNA: 3'- -UGG-UUUUUUGauu-GGUGAGGa--------UGGCA- -5' |
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13111 | 5' | -46.3 | NC_003401.1 | + | 63966 | 0.66 | 0.999898 |
Target: 5'- gGCgGAGAAGCUGACgGCUUUUcGCCa- -3' miRNA: 3'- -UGgUUUUUUGAUUGgUGAGGA-UGGca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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