Results 41 - 47 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13130 | 5' | -53.6 | NC_003401.1 | + | 132436 | 0.7 | 0.810388 |
Target: 5'- cGCGGGcCCGGgGggcgcGCGGGggacccucGCCCCGGg -3' miRNA: 3'- aCGCUCaGGCCgUa----UGUUU--------UGGGGCC- -5' |
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13130 | 5' | -53.6 | NC_003401.1 | + | 100108 | 0.7 | 0.801381 |
Target: 5'- cUGCGAG-CCGGCGUAUuguccGAACCUgcaguCGGc -3' miRNA: 3'- -ACGCUCaGGCCGUAUGu----UUUGGG-----GCC- -5' |
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13130 | 5' | -53.6 | NC_003401.1 | + | 126263 | 0.71 | 0.773447 |
Target: 5'- cUGC-AGUggCUGGUGUcCGAAACCCCGGg -3' miRNA: 3'- -ACGcUCA--GGCCGUAuGUUUUGGGGCC- -5' |
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13130 | 5' | -53.6 | NC_003401.1 | + | 107897 | 0.74 | 0.625497 |
Target: 5'- -cCGGGUCCGGCucggccgccccgaGCGuuGCCCCGGu -3' miRNA: 3'- acGCUCAGGCCGua-----------UGUuuUGGGGCC- -5' |
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13130 | 5' | -53.6 | NC_003401.1 | + | 132690 | 0.74 | 0.599506 |
Target: 5'- gGCGGGgcCCGGCGcgggGCGcgaccgaGGGCCCCGGg -3' miRNA: 3'- aCGCUCa-GGCCGUa---UGU-------UUUGGGGCC- -5' |
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13130 | 5' | -53.6 | NC_003401.1 | + | 22746 | 0.77 | 0.459718 |
Target: 5'- uUGCGAGUCCcaaaacgGGCGUgucuaacuACGGucGCCCCGGg -3' miRNA: 3'- -ACGCUCAGG-------CCGUA--------UGUUu-UGGGGCC- -5' |
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13130 | 5' | -53.6 | NC_003401.1 | + | 58184 | 1.1 | 0.003884 |
Target: 5'- uUGCGAGUCCGGCAUACAAAACCCCGGc -3' miRNA: 3'- -ACGCUCAGGCCGUAUGUUUUGGGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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