Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13273 | 5' | -50.5 | NC_003409.1 | + | 23480 | 0.74 | 0.729076 |
Target: 5'- -cGGCGGACCAAUGGGAgcggGGCAGa-- -3' miRNA: 3'- uuUCGUCUGGUUGUCCUa---CUGUCagg -5' |
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13273 | 5' | -50.5 | NC_003409.1 | + | 80612 | 0.74 | 0.729076 |
Target: 5'- --uGguGGCCAACAGGcUGGCcguGUCCu -3' miRNA: 3'- uuuCguCUGGUUGUCCuACUGu--CAGG- -5' |
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13273 | 5' | -50.5 | NC_003409.1 | + | 113868 | 0.76 | 0.632788 |
Target: 5'- uGGGCGGGCCAGgGGGGUagGACGGUgCg -3' miRNA: 3'- uUUCGUCUGGUUgUCCUA--CUGUCAgG- -5' |
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13273 | 5' | -50.5 | NC_003409.1 | + | 8884 | 1.09 | 0.007083 |
Target: 5'- aAAAGCAGACCAACAGGAUGACAGUCCc -3' miRNA: 3'- -UUUCGUCUGGUUGUCCUACUGUCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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