Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13276 | 3' | -54.6 | NC_003409.1 | + | 132209 | 0.66 | 0.935722 |
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Target: 5'- aCAUGaGGUuggugaGAGCCUCGgUGAUGGCAuAGg -3' miRNA: 3'- -GUGC-CCG------CUCGGGGU-ACUACUGUuUC- -5' |
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| 13276 | 3' | -54.6 | NC_003409.1 | + | 39984 | 0.66 | 0.930592 |
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Target: 5'- uGCGGuugGCG-GCCUCGUcGAUGACGAc- -3' miRNA: 3'- gUGCC---CGCuCGGGGUA-CUACUGUUuc -5' |
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| 13276 | 3' | -54.6 | NC_003409.1 | + | 81117 | 0.66 | 0.925213 |
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Target: 5'- -uUGGGUcgGAG-CCCAUGGUGGCAc-- -3' miRNA: 3'- guGCCCG--CUCgGGGUACUACUGUuuc -5' |
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| 13276 | 3' | -54.6 | NC_003409.1 | + | 74056 | 0.66 | 0.921867 |
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Target: 5'- -uUGGGCGAGCgCCggGAggaacgcgcuggccGGCAGAGg -3' miRNA: 3'- guGCCCGCUCGgGGuaCUa-------------CUGUUUC- -5' |
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| 13276 | 3' | -54.6 | NC_003409.1 | + | 30364 | 0.66 | 0.91311 |
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Target: 5'- uGgGGGCGAGCCCUGgcuauacUGAccuuUGGCAGu- -3' miRNA: 3'- gUgCCCGCUCGGGGU-------ACU----ACUGUUuc -5' |
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| 13276 | 3' | -54.6 | NC_003409.1 | + | 93755 | 0.67 | 0.901222 |
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Target: 5'- -uUGGGaucuauCCCCAUGAUGACAAAc -3' miRNA: 3'- guGCCCgcuc--GGGGUACUACUGUUUc -5' |
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| 13276 | 3' | -54.6 | NC_003409.1 | + | 82857 | 0.67 | 0.899265 |
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Target: 5'- gGCGGGaauGAagacauugaaacauGCgCCAUGGUGGCGGGGg -3' miRNA: 3'- gUGCCCg--CU--------------CGgGGUACUACUGUUUC- -5' |
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| 13276 | 3' | -54.6 | NC_003409.1 | + | 109094 | 0.67 | 0.887766 |
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Target: 5'- -cCGuaGCGGGCCCCGUGccugGACGGGGu -3' miRNA: 3'- guGCc-CGCUCGGGGUACua--CUGUUUC- -5' |
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| 13276 | 3' | -54.6 | NC_003409.1 | + | 100670 | 0.68 | 0.865835 |
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Target: 5'- -cCGGGaCGAGCCCCGUGcacACGuAGg -3' miRNA: 3'- guGCCC-GCUCGGGGUACuacUGUuUC- -5' |
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| 13276 | 3' | -54.6 | NC_003409.1 | + | 15596 | 1.09 | 0.00329 |
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Target: 5'- uCACGGGCGAGCCCCAUGAUGACAAAGg -3' miRNA: 3'- -GUGCCCGCUCGGGGUACUACUGUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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