Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13277 | 3' | -56.1 | NC_003409.1 | + | 113193 | 0.66 | 0.88778 |
Target: 5'- aGGGGCGGCagauaccggccUCCGGGaGGCCuuGGa- -3' miRNA: 3'- cCCCCGUCG-----------AGGUCCgCUGGuuUUac -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 105042 | 0.66 | 0.88778 |
Target: 5'- uGGGGGCugguGCUCgucuGGUGGCU--GGUGg -3' miRNA: 3'- -CCCCCGu---CGAGgu--CCGCUGGuuUUAC- -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 123791 | 0.66 | 0.88778 |
Target: 5'- aGGGGGUgacuAGCUucCCAGGCuaggcgGGCCAu---- -3' miRNA: 3'- -CCCCCG----UCGA--GGUCCG------CUGGUuuuac -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 43990 | 0.66 | 0.88778 |
Target: 5'- aGGGGCguagaauccccGGCcaUCCAGuCGACCGAGAc- -3' miRNA: 3'- cCCCCG-----------UCG--AGGUCcGCUGGUUUUac -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 54401 | 0.66 | 0.88076 |
Target: 5'- --uGGUcgAGUaCCAGGCGGCCAGAAa- -3' miRNA: 3'- cccCCG--UCGaGGUCCGCUGGUUUUac -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 9084 | 0.66 | 0.858364 |
Target: 5'- -uGGGCAcgucuGCUCCAGGUugaACCGAAAa- -3' miRNA: 3'- ccCCCGU-----CGAGGUCCGc--UGGUUUUac -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 3022 | 0.66 | 0.858364 |
Target: 5'- aGGGGGCGGCguuUCCAuaugaGGCCAAGu-- -3' miRNA: 3'- -CCCCCGUCG---AGGUccg--CUGGUUUuac -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 121963 | 0.67 | 0.83409 |
Target: 5'- aGGGuGCGGUgcugcCCAGGaCGGCCGGAu-- -3' miRNA: 3'- cCCC-CGUCGa----GGUCC-GCUGGUUUuac -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 100065 | 0.67 | 0.825613 |
Target: 5'- cGGGGCGGC-UguGGC-ACCAAAuuAUGg -3' miRNA: 3'- cCCCCGUCGaGguCCGcUGGUUU--UAC- -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 90391 | 0.67 | 0.808127 |
Target: 5'- uGGGGCA-CUCgAGGC--CCAGAAUGu -3' miRNA: 3'- cCCCCGUcGAGgUCCGcuGGUUUUAC- -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 42714 | 0.67 | 0.808127 |
Target: 5'- aGGGGGCccucgAGCgcugCCauggAGGCGACCuuGGa- -3' miRNA: 3'- -CCCCCG-----UCGa---GG----UCCGCUGGuuUUac -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 97164 | 0.68 | 0.802751 |
Target: 5'- aGGGaGGCAGUgaauauugagaUUCgguuuauccaggccaAGGCGGCCGGGGUGa -3' miRNA: 3'- -CCC-CCGUCG-----------AGG---------------UCCGCUGGUUUUAC- -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 63966 | 0.68 | 0.799136 |
Target: 5'- aGGGGCucaGGCgCCAGGUGuCCAAc--- -3' miRNA: 3'- cCCCCG---UCGaGGUCCGCuGGUUuuac -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 73930 | 0.68 | 0.799135 |
Target: 5'- -cGGGCgaagAGCuUCCGGGCGgucaugacggagACCAAGGUGu -3' miRNA: 3'- ccCCCG----UCG-AGGUCCGC------------UGGUUUUAC- -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 75464 | 0.68 | 0.789989 |
Target: 5'- aGGGGGguGUUauagGGGCGGCUggGAc- -3' miRNA: 3'- -CCCCCguCGAgg--UCCGCUGGuuUUac -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 131221 | 0.68 | 0.780698 |
Target: 5'- cGGGGCAcuGCUcCCAGGCaGcACCAAc--- -3' miRNA: 3'- cCCCCGU--CGA-GGUCCG-C-UGGUUuuac -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 103638 | 0.68 | 0.780698 |
Target: 5'- aGGGGGCGGCUggcgggUCGGGUGuCCu----- -3' miRNA: 3'- -CCCCCGUCGA------GGUCCGCuGGuuuuac -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 134467 | 0.68 | 0.77127 |
Target: 5'- cGGGGGCGGUgcgcccUCUAGGCauGGCCu----- -3' miRNA: 3'- -CCCCCGUCG------AGGUCCG--CUGGuuuuac -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 24738 | 0.68 | 0.75107 |
Target: 5'- aGGGGGCgcgccggGGCUCCugGGGUGcuGCCGGGGc- -3' miRNA: 3'- -CCCCCG-------UCGAGG--UCCGC--UGGUUUUac -5' |
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13277 | 3' | -56.1 | NC_003409.1 | + | 19651 | 0.69 | 0.742263 |
Target: 5'- -aGGGCAcGCgUCCAGGCaACCGGGGUc -3' miRNA: 3'- ccCCCGU-CG-AGGUCCGcUGGUUUUAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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