Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13278 | 5' | -56.3 | NC_003409.1 | + | 112941 | 0.66 | 0.855551 |
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Target: 5'- -gGCC-CUUGCcaagcGGGCGCGUuGCGCUc -3' miRNA: 3'- uaUGGaGAGCGc----UUCGCGCAuCGCGA- -5' |
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| 13278 | 5' | -56.3 | NC_003409.1 | + | 111385 | 0.66 | 0.84754 |
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Target: 5'- uUGCCUCUCcCGAcGGuUGCGUGGgCGCc -3' miRNA: 3'- uAUGGAGAGcGCU-UC-GCGCAUC-GCGa -5' |
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| 13278 | 5' | -56.3 | NC_003409.1 | + | 127108 | 0.66 | 0.84754 |
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Target: 5'- --uCCUCUCGUuaaGGGCGCGccGGUGCUc -3' miRNA: 3'- uauGGAGAGCGc--UUCGCGCa-UCGCGA- -5' |
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| 13278 | 5' | -56.3 | NC_003409.1 | + | 132796 | 0.67 | 0.822323 |
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Target: 5'- uUACCUggcaCGCGcacAGGCGCGcGGCGCg -3' miRNA: 3'- uAUGGAga--GCGC---UUCGCGCaUCGCGa -5' |
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| 13278 | 5' | -56.3 | NC_003409.1 | + | 83851 | 0.68 | 0.737987 |
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Target: 5'- -cGCCgcaaagUCUCGCGggGacgggGCGUGGgGCUc -3' miRNA: 3'- uaUGG------AGAGCGCuuCg----CGCAUCgCGA- -5' |
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| 13278 | 5' | -56.3 | NC_003409.1 | + | 33878 | 0.68 | 0.728012 |
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Target: 5'- -aGCCaUCauugCGUGAAGCuuGUGGCGCUg -3' miRNA: 3'- uaUGG-AGa---GCGCUUCGcgCAUCGCGA- -5' |
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| 13278 | 5' | -56.3 | NC_003409.1 | + | 109723 | 0.69 | 0.707815 |
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Target: 5'- -cGCUUCUgGCGuGGCGaaGUGGCGCa -3' miRNA: 3'- uaUGGAGAgCGCuUCGCg-CAUCGCGa -5' |
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| 13278 | 5' | -56.3 | NC_003409.1 | + | 22179 | 0.7 | 0.614713 |
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Target: 5'- uUACCgg-CGCGAAGCGCuacGCGCUu -3' miRNA: 3'- uAUGGagaGCGCUUCGCGcauCGCGA- -5' |
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| 13278 | 5' | -56.3 | NC_003409.1 | + | 22354 | 1.05 | 0.003717 |
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Target: 5'- gAUACCUCUCGCGAAGCGCGUAGCGCUu -3' miRNA: 3'- -UAUGGAGAGCGCUUCGCGCAUCGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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