Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13282 | 5' | -54.3 | NC_003409.1 | + | 78805 | 0.66 | 0.926391 |
Target: 5'- gCCUCCGGCugGCGcguaacggcaggcguGAgagGCAaacagaaguggguUCCGACAAg -3' miRNA: 3'- -GGAGGUCGugCGU---------------CU---CGU-------------AGGUUGUU- -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 99473 | 0.66 | 0.930196 |
Target: 5'- aCUCCAGCugaGCGCAGuauuGGCGUggCgCAGCAc -3' miRNA: 3'- gGAGGUCG---UGCGUC----UCGUA--G-GUUGUu -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 119829 | 0.66 | 0.930196 |
Target: 5'- cUCUCuCAGuCGCGCcuAGCugcuUCCAACAGa -3' miRNA: 3'- -GGAG-GUC-GUGCGucUCGu---AGGUUGUU- -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 97288 | 0.66 | 0.930196 |
Target: 5'- aCUCCAGCuucuCGCaaAGGGCgggGUCCAAa-- -3' miRNA: 3'- gGAGGUCGu---GCG--UCUCG---UAGGUUguu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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