Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13283 | 5' | -56.5 | NC_003409.1 | + | 56061 | 0.67 | 0.81043 |
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Target: 5'- -uGGGCGuCCACAUGG-CAGcugaaUCAGCa -3' miRNA: 3'- cgUCCGC-GGUGUGCCaGUCaa---GGUCG- -5' |
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| 13283 | 5' | -56.5 | NC_003409.1 | + | 16633 | 0.68 | 0.764641 |
|
Target: 5'- cGCuGGCcCCGCGCGGccgcCAGguagUCUGGCa -3' miRNA: 3'- -CGuCCGcGGUGUGCCa---GUCa---AGGUCG- -5' |
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| 13283 | 5' | -56.5 | NC_003409.1 | + | 8750 | 0.68 | 0.755093 |
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Target: 5'- cGCAGGCGCgGCGCacucgaGGggugacaccuuUCAGacgUCCAGUu -3' miRNA: 3'- -CGUCCGCGgUGUG------CC-----------AGUCa--AGGUCG- -5' |
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| 13283 | 5' | -56.5 | NC_003409.1 | + | 119810 | 0.71 | 0.613123 |
|
Target: 5'- cGUGGGCGCCGCGCGcGugcucucUCAGUcgcgCCuAGCu -3' miRNA: 3'- -CGUCCGCGGUGUGC-C-------AGUCAa---GG-UCG- -5' |
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| 13283 | 5' | -56.5 | NC_003409.1 | + | 107758 | 0.74 | 0.429214 |
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Target: 5'- uGCAGGCacugaggcuuaGCCACGCGccCAG-UCCGGCg -3' miRNA: 3'- -CGUCCG-----------CGGUGUGCcaGUCaAGGUCG- -5' |
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| 13283 | 5' | -56.5 | NC_003409.1 | + | 110485 | 0.76 | 0.33357 |
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Target: 5'- aGguGaGCGCCACAUGGUCugacaucuucuccagGGUUCCcgAGCg -3' miRNA: 3'- -CguC-CGCGGUGUGCCAG---------------UCAAGG--UCG- -5' |
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| 13283 | 5' | -56.5 | NC_003409.1 | + | 6397 | 0.66 | 0.88688 |
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Target: 5'- cGgGGGUGCCAUggACGGccgaggguguggccUCGGUcaUCCAGg -3' miRNA: 3'- -CgUCCGCGGUG--UGCC--------------AGUCA--AGGUCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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