Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13284 | 3' | -62.7 | NC_003409.1 | + | 131700 | 0.66 | 0.60758 |
Target: 5'- uGCGGgcGGC---CAGGGaCUACCGCUGc -3' miRNA: 3'- -UGCCaaCCGugcGUCCC-GGUGGCGGC- -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 70536 | 0.66 | 0.607579 |
Target: 5'- cUGGUggGGCGCgGCAGcugacucaaaaaGGUCACUGCCu -3' miRNA: 3'- uGCCAa-CCGUG-CGUC------------CCGGUGGCGGc -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 101078 | 0.66 | 0.587923 |
Target: 5'- cACGGUUGGCuCGCugcauGaGGCCuugcggcaGgCGCCGu -3' miRNA: 3'- -UGCCAACCGuGCGu----C-CCGG--------UgGCGGC- -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 13026 | 0.66 | 0.587922 |
Target: 5'- cGCGGUUaGCGgGUaucagGGGGCCACUGUa- -3' miRNA: 3'- -UGCCAAcCGUgCG-----UCCCGGUGGCGgc -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 96441 | 0.66 | 0.578136 |
Target: 5'- -gGGgaGGCAUGCgauacgGGGGCCcaACCGCa- -3' miRNA: 3'- ugCCaaCCGUGCG------UCCCGG--UGGCGgc -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 133599 | 0.66 | 0.568384 |
Target: 5'- aACGccuGUcUGGCACaguucCAGGGCCACCGUUc -3' miRNA: 3'- -UGC---CA-ACCGUGc----GUCCCGGUGGCGGc -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 105054 | 0.66 | 0.562554 |
Target: 5'- cGCGGUgaugugugggGGCugGUgcucgucugguggcuGGuGGCCACCGCg- -3' miRNA: 3'- -UGCCAa---------CCGugCG---------------UC-CCGGUGGCGgc -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 70783 | 0.66 | 0.558675 |
Target: 5'- gACGGUUGGaguCAUGCAuGGCCAgUGUCc -3' miRNA: 3'- -UGCCAACC---GUGCGUcCCGGUgGCGGc -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 107809 | 0.66 | 0.558675 |
Target: 5'- cACGGUgGGCACGCcccuGGaGCUcCCuGCCa -3' miRNA: 3'- -UGCCAaCCGUGCGu---CC-CGGuGG-CGGc -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 128046 | 0.66 | 0.547087 |
Target: 5'- cGCGGUUGGgggugccCGCGUucaGGGGCCcaugcggggcucuGCUGCCc -3' miRNA: 3'- -UGCCAACC-------GUGCG---UCCCGG-------------UGGCGGc -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 33987 | 0.67 | 0.539404 |
Target: 5'- -gGGUacgGGCGCGcCAGGGaCAgCGCCc -3' miRNA: 3'- ugCCAa--CCGUGC-GUCCCgGUgGCGGc -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 51487 | 0.67 | 0.529852 |
Target: 5'- cCGGUUcagccGGuCGC-CAGGGUCAucCCGCCGg -3' miRNA: 3'- uGCCAA-----CC-GUGcGUCCCGGU--GGCGGC- -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 97993 | 0.67 | 0.509066 |
Target: 5'- gACGGcucuucagugccUGGCAgaucCAGGGUCACCGCCu -3' miRNA: 3'- -UGCCa-----------ACCGUgc--GUCCCGGUGGCGGc -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 16791 | 0.67 | 0.498801 |
Target: 5'- cUGGc-GGcCGCGCGGGGCCAgcguaauuuuugguCCGCUGu -3' miRNA: 3'- uGCCaaCC-GUGCGUCCCGGU--------------GGCGGC- -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 133203 | 0.68 | 0.483123 |
Target: 5'- cCGGUggugaGGCACcuGCAGGcGCUGgCGCCGc -3' miRNA: 3'- uGCCAa----CCGUG--CGUCC-CGGUgGCGGC- -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 87346 | 0.68 | 0.474012 |
Target: 5'- -aGGgUGGCGCGUc-GGCCAcgcccuCCGCCGg -3' miRNA: 3'- ugCCaACCGUGCGucCCGGU------GGCGGC- -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 121978 | 0.68 | 0.464988 |
Target: 5'- cGCGGUUGuuUACGCAGGGUgcggUGCUGCCc -3' miRNA: 3'- -UGCCAACc-GUGCGUCCCG----GUGGCGGc -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 130623 | 0.68 | 0.441956 |
Target: 5'- -gGGUuucugggauagugucUGGCugGCAGGGUCucauCCGCgGc -3' miRNA: 3'- ugCCA---------------ACCGugCGUCCCGGu---GGCGgC- -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 55449 | 0.69 | 0.42128 |
Target: 5'- aGCGaaUGGCGgGCGGcaaGCCGCCGCCc -3' miRNA: 3'- -UGCcaACCGUgCGUCc--CGGUGGCGGc -5' |
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13284 | 3' | -62.7 | NC_003409.1 | + | 67802 | 0.7 | 0.380142 |
Target: 5'- cGCGGUgggugcGGCGCGCAGGG--GgCGCCa -3' miRNA: 3'- -UGCCAa-----CCGUGCGUCCCggUgGCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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