Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13290 | 5' | -47.7 | NC_003409.1 | + | 107947 | 0.66 | 0.998608 |
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Target: 5'- --uGGCCUGCUGcaaAUCGC-CGGaCUGGAg -3' miRNA: 3'- gauCUGGACGAU---UAGUGaGUC-GAUUU- -5' |
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| 13290 | 5' | -47.7 | NC_003409.1 | + | 119269 | 0.66 | 0.998608 |
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Target: 5'- -aGGGgCUGC-GAUgACUCAGCUGc- -3' miRNA: 3'- gaUCUgGACGaUUAgUGAGUCGAUuu -5' |
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| 13290 | 5' | -47.7 | NC_003409.1 | + | 113338 | 0.67 | 0.997703 |
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Target: 5'- --uGACCUGCUGuucgaagcccccaccGUCGCUacccuGGCUAAGg -3' miRNA: 3'- gauCUGGACGAU---------------UAGUGAg----UCGAUUU- -5' |
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| 13290 | 5' | -47.7 | NC_003409.1 | + | 91772 | 0.69 | 0.988046 |
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Target: 5'- cCUAGGCg-GUUGGgauUCACUCAGCUGc- -3' miRNA: 3'- -GAUCUGgaCGAUU---AGUGAGUCGAUuu -5' |
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| 13290 | 5' | -47.7 | NC_003409.1 | + | 23742 | 0.69 | 0.986348 |
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Target: 5'- aUAGGgCUGC-GAUgACUCAGCUGc- -3' miRNA: 3'- gAUCUgGACGaUUAgUGAGUCGAUuu -5' |
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| 13290 | 5' | -47.7 | NC_003409.1 | + | 21936 | 0.7 | 0.980139 |
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Target: 5'- -gGGcauCCUGCUcGUCGCUguGCUGAc -3' miRNA: 3'- gaUCu--GGACGAuUAGUGAguCGAUUu -5' |
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| 13290 | 5' | -47.7 | NC_003409.1 | + | 35210 | 0.7 | 0.980139 |
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Target: 5'- -aAGACUUGUcugAGUCAC-CAGCUGAu -3' miRNA: 3'- gaUCUGGACGa--UUAGUGaGUCGAUUu -5' |
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| 13290 | 5' | -47.7 | NC_003409.1 | + | 8844 | 0.71 | 0.965454 |
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Target: 5'- uCUAGACCUGggAGUCauugugguuGCUCAGUUGc- -3' miRNA: 3'- -GAUCUGGACgaUUAG---------UGAGUCGAUuu -5' |
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| 13290 | 5' | -47.7 | NC_003409.1 | + | 34176 | 1.07 | 0.016179 |
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Target: 5'- gCUAGACCUGCUAAUCACUCAGCUAAAa -3' miRNA: 3'- -GAUCUGGACGAUUAGUGAGUCGAUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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