Results 61 - 64 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13291 | 3' | -52.5 | NC_003409.1 | + | 27421 | 0.73 | 0.649 |
Target: 5'- cGGgUUCCAGCAGCAcccacCGCccguccaaauucuAAAAGAGUg -3' miRNA: 3'- uCCgAAGGUCGUCGU-----GCG-------------UUUUCUCG- -5' |
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13291 | 3' | -52.5 | NC_003409.1 | + | 109674 | 0.76 | 0.492427 |
Target: 5'- gAGGCUUCCAGCgugagguacGGCACaGCcuu-GAGCa -3' miRNA: 3'- -UCCGAAGGUCG---------UCGUG-CGuuuuCUCG- -5' |
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13291 | 3' | -52.5 | NC_003409.1 | + | 131110 | 0.76 | 0.492427 |
Target: 5'- uGGGCUUCCAGCuGCugGUGGcccuGGGUg -3' miRNA: 3'- -UCCGAAGGUCGuCGugCGUUuu--CUCG- -5' |
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13291 | 3' | -52.5 | NC_003409.1 | + | 35445 | 1.13 | 0.00244 |
Target: 5'- cAGGCUUCCAGCAGCACGCAAAAGAGCa -3' miRNA: 3'- -UCCGAAGGUCGUCGUGCGUUUUCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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