Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13291 | 5' | -63.4 | NC_003409.1 | + | 35267 | 0.66 | 0.572165 |
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Target: 5'- uGGGCCCgguuACCU-GCUcuuuuGCgUGCUGCUGGa -3' miRNA: 3'- -CCCGGG----UGGAcCGG-----UGgAUGGCGGCC- -5' |
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| 13291 | 5' | -63.4 | NC_003409.1 | + | 24736 | 0.72 | 0.279621 |
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Target: 5'- gGGGCgCGCCggGGCU-CCUgggguGCUGCCGGg -3' miRNA: 3'- -CCCGgGUGGa-CCGGuGGA-----UGGCGGCC- -5' |
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| 13291 | 5' | -63.4 | NC_003409.1 | + | 23799 | 0.68 | 0.443736 |
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Target: 5'- uGGGuucaCCCACCUGGguaUACCUACUggauuacccaGCUGGg -3' miRNA: 3'- -CCC----GGGUGGACCg--GUGGAUGG----------CGGCC- -5' |
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| 13291 | 5' | -63.4 | NC_003409.1 | + | 23196 | 0.66 | 0.58178 |
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Target: 5'- cGGGUgCACggGGUCACCUccaggucacGCCGCUaGGu -3' miRNA: 3'- -CCCGgGUGgaCCGGUGGA---------UGGCGG-CC- -5' |
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| 13291 | 5' | -63.4 | NC_003409.1 | + | 18940 | 0.71 | 0.292328 |
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Target: 5'- cGGGgCCAUCuUGGUCGCCUGgacugcccCCGCgGGg -3' miRNA: 3'- -CCCgGGUGG-ACCGGUGGAU--------GGCGgCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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