Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13291 | 5' | -63.4 | NC_003409.1 | + | 67977 | 0.69 | 0.393792 |
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Target: 5'- aGGGCCCuCgUGGCgcccCCUGCgCGCCGc -3' miRNA: 3'- -CCCGGGuGgACCGgu--GGAUG-GCGGCc -5' |
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| 13291 | 5' | -63.4 | NC_003409.1 | + | 90446 | 0.69 | 0.410035 |
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Target: 5'- aGGGCUCAauc-GCCGCCUcgGCUGUCGGg -3' miRNA: 3'- -CCCGGGUggacCGGUGGA--UGGCGGCC- -5' |
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| 13291 | 5' | -63.4 | NC_003409.1 | + | 74162 | 0.68 | 0.443736 |
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Target: 5'- uGGGUguugUCGCCUcuGCCGCCguaGCCGCCGc -3' miRNA: 3'- -CCCG----GGUGGAc-CGGUGGa--UGGCGGCc -5' |
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| 13291 | 5' | -63.4 | NC_003409.1 | + | 23799 | 0.68 | 0.443736 |
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Target: 5'- uGGGuucaCCCACCUGGguaUACCUACUggauuacccaGCUGGg -3' miRNA: 3'- -CCC----GGGUGGACCg--GUGGAUGG----------CGGCC- -5' |
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| 13291 | 5' | -63.4 | NC_003409.1 | + | 110087 | 0.66 | 0.601102 |
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Target: 5'- -cGCCagagcguuccugCGCCUGGCCgACCaggcGCuCGCCGGu -3' miRNA: 3'- ccCGG------------GUGGACCGG-UGGa---UG-GCGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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