Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13292 | 3' | -55.8 | NC_003409.1 | + | 38618 | 0.66 | 0.880684 |
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Target: 5'- uGCGCGCCGa-GGCgCCGCAgucGUCcGCu -3' miRNA: 3'- -UGCGCGGUcaCCGaGGUGUa--CAGuUG- -5' |
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| 13292 | 3' | -55.8 | NC_003409.1 | + | 11815 | 0.67 | 0.825004 |
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Target: 5'- cCGUGUaGGUGGuCUCCACgAUGUCGu- -3' miRNA: 3'- uGCGCGgUCACC-GAGGUG-UACAGUug -5' |
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| 13292 | 3' | -55.8 | NC_003409.1 | + | 107650 | 0.67 | 0.81626 |
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Target: 5'- cCGCuGCCAG-GGCaUCC-CA-GUCAGCg -3' miRNA: 3'- uGCG-CGGUCaCCG-AGGuGUaCAGUUG- -5' |
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| 13292 | 3' | -55.8 | NC_003409.1 | + | 20199 | 0.67 | 0.807341 |
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Target: 5'- uGCGCGUCagcugcagccguAGUGGCUCUAUAUG-CGu- -3' miRNA: 3'- -UGCGCGG------------UCACCGAGGUGUACaGUug -5' |
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| 13292 | 3' | -55.8 | NC_003409.1 | + | 78671 | 0.68 | 0.760452 |
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Target: 5'- gACGaCcCCAGccccuUGGUUCCACGaaUGUCAGCg -3' miRNA: 3'- -UGC-GcGGUC-----ACCGAGGUGU--ACAGUUG- -5' |
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| 13292 | 3' | -55.8 | NC_003409.1 | + | 21173 | 0.7 | 0.679796 |
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Target: 5'- cCGUGCCAGggGGUUUCGCuucUGUCAAa -3' miRNA: 3'- uGCGCGGUCa-CCGAGGUGu--ACAGUUg -5' |
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| 13292 | 3' | -55.8 | NC_003409.1 | + | 24734 | 0.72 | 0.534878 |
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Target: 5'- gGCGCGCCGG-GGCUCCugGgGUg--- -3' miRNA: 3'- -UGCGCGGUCaCCGAGGugUaCAguug -5' |
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| 13292 | 3' | -55.8 | NC_003409.1 | + | 24068 | 0.72 | 0.52481 |
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Target: 5'- gGCGCGCCccagaacaugGGUGGCUaacgcCUACAUGggCAGCu -3' miRNA: 3'- -UGCGCGG----------UCACCGA-----GGUGUACa-GUUG- -5' |
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| 13292 | 3' | -55.8 | NC_003409.1 | + | 37289 | 1.1 | 0.002032 |
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Target: 5'- aACGCGCCAGUGGCUCCACAUGUCAACg -3' miRNA: 3'- -UGCGCGGUCACCGAGGUGUACAGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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