Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13292 | 5' | -58.2 | NC_003409.1 | + | 71125 | 0.65 | 0.791848 |
Target: 5'- -aGCCAGCGuaUGCuucaggaccaccAGGGaCGCUAAGAa -3' miRNA: 3'- agCGGUCGU--ACGu-----------UCCCgGCGGUUCU- -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 39548 | 0.66 | 0.784491 |
Target: 5'- gCGCCgcauGGCuagGUGCAGGGGUgGUCGAa- -3' miRNA: 3'- aGCGG----UCG---UACGUUCCCGgCGGUUcu -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 111997 | 0.66 | 0.784491 |
Target: 5'- gUGCCGG-AUGC-AGGGCCugGCCucGAa -3' miRNA: 3'- aGCGGUCgUACGuUCCCGG--CGGuuCU- -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 75668 | 0.66 | 0.784491 |
Target: 5'- gUGCCAGCA-GCcAGcGUCGCCAacGGAc -3' miRNA: 3'- aGCGGUCGUaCGuUCcCGGCGGU--UCU- -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 12013 | 0.66 | 0.784491 |
Target: 5'- -aGCCAGCcUG-GAGGGCCuGCUggAGGAc -3' miRNA: 3'- agCGGUCGuACgUUCCCGG-CGG--UUCU- -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 77091 | 0.66 | 0.765721 |
Target: 5'- cCGCUAcuguGgGUGCAcugagugcGGcGGCCGCUAAGAa -3' miRNA: 3'- aGCGGU----CgUACGU--------UC-CCGGCGGUUCU- -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 131819 | 0.66 | 0.765721 |
Target: 5'- -gGCCGGC-UGCAGGGGaCUGCa---- -3' miRNA: 3'- agCGGUCGuACGUUCCC-GGCGguucu -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 114396 | 0.66 | 0.756152 |
Target: 5'- gCGaCAGCggGguGGGGCUGgCAAGGc -3' miRNA: 3'- aGCgGUCGuaCguUCCCGGCgGUUCU- -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 95232 | 0.67 | 0.715862 |
Target: 5'- cCGCUAGCcaccgcaacggcgGUGUAucAGGcGCCGCCAAu- -3' miRNA: 3'- aGCGGUCG-------------UACGU--UCC-CGGCGGUUcu -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 118463 | 0.67 | 0.710852 |
Target: 5'- gCGCCggguuccuggggugcGGgGUGCGGGGGaccgCGCCGGGGu -3' miRNA: 3'- aGCGG---------------UCgUACGUUCCCg---GCGGUUCU- -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 43097 | 0.67 | 0.706832 |
Target: 5'- aCGCaAGCGgcgGCGAGGGCgGUCuccAGAa -3' miRNA: 3'- aGCGgUCGUa--CGUUCCCGgCGGu--UCU- -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 16632 | 0.67 | 0.706832 |
Target: 5'- aCGCUGGCcccGCGc-GGCCGCCAGGu -3' miRNA: 3'- aGCGGUCGua-CGUucCCGGCGGUUCu -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 19367 | 0.67 | 0.706832 |
Target: 5'- -aGCCgaggcaauAGCGUcauuucgcGCAAGGGUCGCCAGa- -3' miRNA: 3'- agCGG--------UCGUA--------CGUUCCCGGCGGUUcu -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 94671 | 0.68 | 0.676398 |
Target: 5'- cUCGUgAGCAUGCu--GGCCGCaauGGGg -3' miRNA: 3'- -AGCGgUCGUACGuucCCGGCGgu-UCU- -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 133036 | 0.68 | 0.676398 |
Target: 5'- gCGCCAGCGccUGCAGGuGCCucaccaccgggGCCGGGu -3' miRNA: 3'- aGCGGUCGU--ACGUUCcCGG-----------CGGUUCu -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 111850 | 0.68 | 0.635345 |
Target: 5'- gCGUCGGCcaccGCGGGGGCgGCCGu-- -3' miRNA: 3'- aGCGGUCGua--CGUUCCCGgCGGUucu -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 43641 | 0.68 | 0.625055 |
Target: 5'- aCGCCAGCuacguUGUcAGGGgUGCCAAc- -3' miRNA: 3'- aGCGGUCGu----ACGuUCCCgGCGGUUcu -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 113095 | 0.68 | 0.625055 |
Target: 5'- gCGaCgGGUGUGCGAGGGCaccuCCAGGAg -3' miRNA: 3'- aGC-GgUCGUACGUUCCCGgc--GGUUCU- -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 70207 | 0.69 | 0.61477 |
Target: 5'- aUGCCGGgGUGCGcgGGGGUC-CCGAGu -3' miRNA: 3'- aGCGGUCgUACGU--UCCCGGcGGUUCu -5' |
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13292 | 5' | -58.2 | NC_003409.1 | + | 133702 | 0.69 | 0.61477 |
Target: 5'- cCGCCAGCGguaGCAGGucuGCCcCCAGGGa -3' miRNA: 3'- aGCGGUCGUa--CGUUCc--CGGcGGUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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