Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13293 | 3' | -56.2 | NC_003409.1 | + | 113869 | 0.66 | 0.896739 |
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Target: 5'- uUGGGCggGC-CAGGgGgGuaggacGGUGCGGa -3' miRNA: 3'- -ACCCGuaCGuGUCUgCgCu-----CUACGCC- -5' |
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| 13293 | 3' | -56.2 | NC_003409.1 | + | 117156 | 0.66 | 0.883161 |
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Target: 5'- uUGcGGCGcuagacUGCACcGA--CGAGAUGCGGg -3' miRNA: 3'- -AC-CCGU------ACGUGuCUgcGCUCUACGCC- -5' |
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| 13293 | 3' | -56.2 | NC_003409.1 | + | 118467 | 0.72 | 0.573837 |
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Target: 5'- cGGGUuc-CugGGGUGCGGGGUGCGGg -3' miRNA: 3'- aCCCGuacGugUCUGCGCUCUACGCC- -5' |
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| 13293 | 3' | -56.2 | NC_003409.1 | + | 121963 | 0.67 | 0.828839 |
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Target: 5'- aGGGUgcgGUGCugcccaGGACgGCcGGAUGCGGg -3' miRNA: 3'- aCCCG---UACGug----UCUG-CGcUCUACGCC- -5' |
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| 13293 | 3' | -56.2 | NC_003409.1 | + | 132801 | 0.66 | 0.861119 |
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Target: 5'- -uGGCAcgcGCACAGGCGCGcGgcGCGc -3' miRNA: 3'- acCCGUa--CGUGUCUGCGCuCuaCGCc -5' |
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| 13293 | 3' | -56.2 | NC_003409.1 | + | 133546 | 0.7 | 0.645633 |
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Target: 5'- aGGGCGUacaGCAGGC-CGAGAUGCu- -3' miRNA: 3'- aCCCGUAcg-UGUCUGcGCUCUACGcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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